Sol-gel法固定化漆酶处理氯酚类污染物  

Chlorophenols treatment with immobilization of laccase by Sol-gel

作　　者：邱凌峰[1] 黄志心[1] 

机构地区：[1]福州大学环境与资源学院,福建福州350002

出　　处：《福州大学学报（自然科学版）》2008年第6期910-914,共5页Journal of Fuzhou University(Natural Science Edition)

基　　金：福建省重大科技计划资助项目(2006SZ010080044)

摘　　要：以2,4-二氯酚(2,4-DCP)和2,4,6-三氯酚(2,4,6-TCP)为代表,采用Sol-gel法固定化漆酶处理氯酚类污染物.结果表明:底物初始浓度为0.5 mmol/L,固定化漆酶投加量为7 g/L,反应3 h后DCP去除率可达95.3%,而反应2 h后TCP去除率可达99.6%.TCP和DCP去除过程可以用米氏方程描述,其米氏常数Km值分别为11.72和9.97 mmol/L,因此固定化漆酶对TCP的亲和力高于DCP,这导致了在反应时间、初始浓度、固定化酶投加量以及底物共存等因素变化后两者去除效果上的差异.连续48 h的运行数据和酶活损失表明固定化漆酶具有较好的反应稳定性.2, 4 - DCP and 2, 4, 6 - TCP chosen as model of chlorophenols, the treatment process of them with immobilized laccase by Sol- gel showed good removal effect and response stability. On the condition of the initial substrate concentration of 0.5 mmol/L and immobilized laccase dosage to 7g/L, DCP removal rate up to 95.3% after 3 hours and TCP removal rate up to 99.6% reaction after 2h, showed good treatment effect. The degradation procession of them could be described with Michaelis - Menton Formulation with Km value of DCP and TCP being 11.72 and 9.97 mmol/L respectively. The affinity of TCP with immobilization of Laccase was higher than its of DCP, which leaded to different of removal effect under the variable conditions, i. e. , reaction time, initial concentration, dosage of immobilized laccase and mixture. Data of continuous operation within 48 hours and the loss activity of immobilized laccase showed its good reaction stability.

关 键 词：SOL-GEL法 固定化漆酶 氯酚类污染物 

分 类 号：X703.1[环境科学与工程—环境工程]