稀土离子增敏的恩诺沙星荧光体系及其分析应用  

Fluorescence Characteristic of Enro Floxacin Sensitized by Rare Earth Ion and Its Analytical Application

作　　者：卓霞军[1] 童裳伦[1] 

机构地区：[1]浙江大学环境科学研究所,浙江杭州310029

出　　处：《光谱学与光谱分析》2008年第12期2939-2943,共5页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(20577039);浙江省自然科学基金杰出青年团项目(R507212)资助

摘　　要：研究了稀土Y^3＋离子与恩诺沙星配合物体系的荧光特性,Y^3＋离子可使体系的荧光强度明显增强,由此建立了灵敏测定恩诺沙星药物的分析方法。用1 cm石英比色池在激发和发射波长分别为274和424 nm处测定其荧光强度;恩诺沙星在1.0×10^-9-5.0×10^-6mol·L^-1浓度范围内与体系的荧光强度呈良好的线性关系,相关系数为0.998 1,检测限为2.3×10^-10mol·L^-1（S/N=3）;同时试验了常见金属离子与配伍药对其测定的干扰,进行了在配伍药中的回收试验,回收率在98.0%-107.0%之间,RSD在0.9%-4.5%之间以及对动物专用的恩诺沙星注射液的含量进行了测定,并与用铽离子荧光探针法测得的结果进行了比较,结果令人满意。此外,对该荧光体系的发光机理也进行了讨论。The fluorescence characteristic of enrofloxacin-yttrium（Ⅲ）（ENRX-Y^3＋） was investigated in the present paper.Experimental results indicated that Y^3＋ ion could greatly increase the fluorescence intensity of the system.From this,a simple and sensitive method of determining the ENRX was set up.The fluorescence intensity of the system was measured with a 1 cm quartz cell at the excitation wavelengths of 274 nm and the emission wavelengths of 424 nm.The optimal conditions were obtained as follows： pH=5.56.2,0.1 mol·L^-1 hexamethylenamine（HMA） buffer,the concentration of yttrium（Ⅲ） was 1.0×10^-4 mol·L- 1.Under the optimal conditions,the fluorescence intensity of the system showed a good linear relationship with the concentration of ENRX in the range of 1.0×10^-9-5.0×10^-6 mol·L^-1,its correlation coefficient was 0.998 1,and the detection limit（S/N=3） was 2.3×10^-10 mol·L- 1.Interferences of common metal ions and other pharmaceuticals usually used for the compatibility of medicines with the fluorescence intensity of the system were studied,and the results showed that most metal ions except Tb^3＋,Cu^2＋ and Al^3＋ and the compatible medicines used together with ENRX did not interfere with its determination.The recovery tests in the compatible medicines were also performed,and the results showed that all the recoveries were in the range of 98.0%-107.0%,and RSDs were in the range of 0.9%-4.5%.The presented method was used to determine the ENRX in actual pharmaceutical samples which were used in the treatment for animals as compared with the reported method of terbium（Ⅲ） luminescence probe,and the results were satisfactory.In addition,the luminescence mechanism of the ENRX-Y^3＋ system was also discussed.

关 键 词：恩诺沙星 氟喹诺酮 钇(Ⅲ)离子 荧光分析法 测定 

分 类 号：O561.3[理学—原子与分子物理]