大鼠单根近球肾小管钠泵活性测定的改良方法  

An improved method of determining of sodium pump activity in single proximal tubules of the rat

作　　者：罗蕾[1] 刘红[2] 余德芊[1] 刘爱东[1] 高原[3] 

机构地区：[1]遵义医学院生理学教研室,贵州遵义563003 [2]川北医学院生理学教研室,四川南充637007 [3]遵义医学院珠海校区生理学教研室,广东珠海519041

出　　处：《四川生理科学杂志》2008年第4期156-158,共3页Sichuan Journal of Physiological Sciences

基　　金：珠海市科技基金资助课题(PC2003010057)

摘　　要：目的:以Doucet介绍的方法为基础,改良了测定大鼠肾脏近端小管Na+-K+-ATPase活性的方法。方法:首先,利用自制的玻璃分针能准确而迅速的分离获取近端小管;其次,使用传导性能较好和易于折叠的锡箔纸代替了铝片,简化了孵育和测定过程。结果:改良方法与Doucet建立的经典方法比较,测定结果无显著性差异(P>0.05);但对小管长度确定平均耗时和转移含32P的孵育液平均耗时明显降低。结论:本文介绍的测定大鼠肾脏近端小管Na+-K+-ATPase活性的改良方法,减少操作步骤,缩短了操作时间和暴露在放射性环境中的时间;简化并改进了操作条件,降低了实验要求和成本。Objective： The approach used for measuring Na^＋-K^＋-ATPase activity in single proximal tubules of the rat has been improved. Methods： This method was based on the method reported by Doucet and his colleagues with the following modifications First, the use of stir-made glass microneedle with cusp bent can catch the target tubule accurately and quickly. Second, aluminum plaque was replaced by tinfoil papers which have characteristics of conducting heat quickly and the very thin and flexible. Procedures of measuring have been improved and predigested for using the tinfoil paper. Results. There is no significant difference in results between the method suggested by Doucet et ak and the modified one（P〈0. 05）. Conclusion.. Compared with the method used by Doucet, the modified one has advantage： the experimental procedures, time, operational condition and requirements have been reduced.

关 键 词：近球小管 钠泵 活性测定 改良方法 大鼠 

分 类 号：R692[医药卫生—泌尿科学]