Host cell apoptosis induced by infection with duck swollen head hemorrhagic disease virus  

作　　者：Chuanfeng Li Anchun Cheng Mingshu Wang Chanjuan Shen Na Zhang Yi Zhou Dekang Zhu Qihui Luo Renyong Jia Xiaoyue Chen 

机构地区：[1]Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Ya'an 625014, China [2]Key Laboratory of Animal Disease and Human Health of Sichuan Province, Ya'an 625014, China [3]College of Life Science and Technology of Southwest University for Nationalities, Chengdu 610041, China

出　　处：《Progress in Natural Science:Materials International》2009年第1期47-53,共7页自然科学进展·国际材料（英文版）

基　　金：supported by National Natural Science Foundation of China (Grant No.30471297,30771598);the New Century Excellent Scientist Research Program of China Education Department (No.NCET-04-0906/NCET-06-0818);the National Science and Technology Support Programs (No.2007Z06-017);the Sichuan Excellent Scientist Research Funds (No. 03ZQ026-028,05ZQ026-038,07ZQ026-132);the China University Science and Technology Innovation Key Project Funds of Education Department (No.706050);the Sichuan Key Courses Construction Project (No.SZD0418)

摘　　要：This study aimed to examine the host cell apoptosis in the tissues of Peking ducks infected with duck swollen head hemorrhagic disease virus(DSHDV).The dynamic changes associated with apoptosis occurring in the internal tissues were evaluated at different time points postinoculation(PI) by performing hematoxylin and eosin(HE) staining,followed by light microscopy,terminal deoxynucleotidyl transferase dUTP nick-end labeling(TUNEL) assay,and transmission electron microscopy(TEM).The results showed that DSHDV infection could induce apoptosis in host cells,including those of the bursa of Fabricius(BF),thymus,spleen,liver,intestinal tract,kidney,and esophagus.The apoptotic index(AI) values increased with time from 2 to 72 h PI,and the highest values were recorded at 72 h PI.Further,cell death due to classic necrosis was observed in the dying or deceased ducks after 72 h PI.In conclusion,host cell apoptosis can be induced by DSHDV and may play an important role in the pathogenesis of duck viral swollen head hemorrhagic disease(DVSHD).This study aimed to examine the host cell apoptosis in the tissues of Peking ducks infected with duck swollen head hemorrhagic disease virus （DSHDV）. The dynamic changes associated with apoptosis occurring in the internal tissues were evaluated at different time points postinoculation （PI） by performing hematoxylin and eosin （HE） staining, followed by light microscopy, terminal deoxynucleotidyl transferase dUTP nick-end labeling （TUNEL） assay, and transmission electron microscopy （TEM）. The results showed that DSHDV infection could induce apoptosis in host cells, including those of the bursa of Fabricius （BF）, thymus, spleen, liver, intestinal tract, kidney, and esophagus. The apoptotic index （AI） values increased with time from 2 to 72 h PI, and the highest values were recorded at 72 h PI. Further, cell death due to classic necrosis was observed in the dying or deceased ducks after 72 h PI. In conclusion, host cell apoptosis can be induced by DSHDV and may play an important role in the pathogenesis of duck viral swollen head hemorrhagic disease （DVSHD）.

关 键 词：Duck swollen head hemorrhagic disease virus （DSHDV） Terminal deoxynucleotidyl transferase dUTP nick-end labeling （TUNEL） Host cell APOPTOSIS 

分 类 号：R605.972[医药卫生—急诊医学]