乙酰基蝙蝠葛苏林碱抗缺血性脑损伤作用  

THE ANTIISCHEMIC EFFECT OF O,OACETYLDAURISOLINE

作　　者：王霆[1] 慕海鹰 刘国卿[1] 

机构地区：[1]中国药科大学药理教研室

出　　处：《药学学报》1998年第4期241-244,共4页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金

摘　　要：用细胞培养方法和大鼠脑缺血损伤模型，研究乙酰基蝙蝠葛苏林碱（Ｏ，Ｏａｃｅｔｙｌｄａｕｒｉｓｏｌｉｎｅ，Ａｄａｕ）对缺血性脑损伤的保护作用。结果表明，Ａｄａｕ对低钾、高钾、ＢａｙＫ８６４４及去甲肾上腺素所引起ＰＣ１２细胞内游离钙浓度增加有抑制作用，其ＩＣ５０分别为４６３±０７２，０１５±００２，９３７±１７６和１００±１４μｍｏｌ·Ｌ－１。Ａｄａｕ在整体大鼠双动脉结扎（２５，５，１０ｍｇ·ｋｇ－１，ｉｖ）和四动脉结扎（５，１０ｍｇ·ｋｇ－１，ｉｖ）模型上，均能降低缺血脑组织脂质过氧化物含量，增加超氧物歧化酶（ＳＯＤ）活性。提示Ａｄａｕ对脑缺血损伤有明显的保护作用。O,Oacetyldaurisoline(Adau) is a derivative of daurisoline (Dau). In cultured PC12 cells, Adau was found to inhibit K+, Bay K8644 and norepinephrine induced intracellular free calcium concentration increase. Adau was also shown to protect PC12 cells from hypoxiareoxygenation injury with EC50 of 643±125 μmol·L-1 by MTT assay. Adau (25,5 and 10 mg·kg-1,iv) administrated 30 min before ischemia in bilateral carotid artery occlusion and fourvessel occlusion rats, attenuated the increase of lipid peroxide content and the decrease of SOD activity. These results show that Adau have significant protective effects aganist ischemic injury.

关 键 词：乙酰基 蝙蝠葛苏林碱 脑缺血 中医药疗法 

分 类 号：R277.733.1[医药卫生—中医学] R285.5