饲料维生素E添加量对花鲈生长、组织中维生素E积累量和免疫指标的影响  

Effects of dietary vitamin E supplement on growth,tissue vitam in E concentration and immune responses of Japanese seabass(Lateolabrax japonicus)

作　　者：周立斌[1,2] 张伟[1] 王安利[1] 张海发[3] 刘付永忠[3] 

机构地区：[1]华南师范大学生命科学学院,广东省高等学校生态与环境科学重点实验室,广东广州510631 [2]惠州学院生命科学系,广东惠州516007 [3]广东省大亚湾水产试验中心,广东惠州516081

出　　处：《水产学报》2009年第1期95-102,共8页Journal of Fisheries of China

基　　金：国家科技支撑计划课题(2007BAD29B04);广东省科技计划项目(2007B020708013);广东省重大科技兴海(兴渔)项目(A200501G01)

摘　　要：采用单因子试验设计方法，进行了饲料中添加维生素E对花鲈生长（增重率、存活率、特定生长率、饲料效率）、组织中维生素E积累量和免疫指标（血清中溶菌酶活性和总补体活性）影响的研究，设计了5个不同维生素E水平（0．6、24．7、49．0、98．8、198．2mg／kg）的等氮等能饲料，对花鲈进行为期／3周的生长实验，每个水平含3个重复，每个养殖单元放养初始体重为10．1g左右的花鲈20尾。饲养试验在海水网箱（1．5m×1．0m×1．0m）中进行。结果表明：饲料中未添加维生素E组的增重率、饲料效率、特定生长率以及存活率显著低于维生素E添加组，随着饲料中维生素E添加量由0．6提高到49．0mg／kg时，花鲈的增重率和特定生长率显著提高，以增重率和特定生长率为指标，花鲈最佳生长性能的饲料维生素E添加量为60．5mg／kg左右；对鱼体生物学指标的分析表明，饲料中维生素E添加量达到49mg／kg时能显著影响鱼体的肝体比指数，鱼体水分、粗脂肪不受饲料中维生素E添加水平的影响，然而对鱼体粗蛋白含量的影响显著；随着饲料中维生素E添加量由0．6提高到49．0mg／kg时，花鲈肌肉和肝脏中维生素E的积累量显著升高，但当维生素E添加量达到49．0mg／kg后，肌肉和肝脏中维生素E的积累量变化不显著；饲料中未添加维生素E组的花鲈血清溶菌酶和总补体活性显著低于维生素E添加组，花鲈血清溶菌酶和总补体活性随着维生素E添加量的增加而显著升高，饲料维生素E添加量达到98．8mg／kg时，血清溶菌酶和总补体活性较高。综上所述，花鲈生长和免疫的维生素E适宜添加量为60．5—98．8mg／kg。An experiment was conducted to study the effects of dietary vitamin E on growth （ weight gain rate,feed efficiency ratio, specific growth rate and survival rate）, vitamin E concentration in tissue and immune response （ lysozyme activity and total complement activity ） of Japanese seabass （ Lateolabrax japonicus）. Five iso-nitrogenous and iso-energetic diets were formulated to contain graded level of vitamin E （0.6,24.7,49.0,98.9, and 198.2 mg/kg diet, respectively ） and fed to 15 floating netcages （1.5 m × 1.0 m ×1.0 m） with 20 fish （ initial weight： 10.1 g） each twice daily （09 ： 30,16：30） for 8 weeks. Results indicated that after 8 weeks of the feeding trial, weight gain rate, feed efficiency ratio, specific growth rate and survival rate of the fish fed with the control diet were significantly lower than those with vitamin E supplemented diets. The weight gain rate and specific growth rate increased significantly with increasing dietary vitamin E from 0.6 to 49.0 mg/kg and the optimal vitamin E content for maximum growth is about 60.5 mg; Moisture and fat concentration in whole body of Japanese seabass were not affected by the supplemental levels of dietary vitamin E, but the protein concentration increased significantly with increasing dietary vitamin E from 0.6 to 49.0 mg/kg. Meanwhile, the vitamin E concentration in liver and musle increased significantly with increasing dietary vitamin E from 0. 6 to 49.0 mg/kg, but no significant differences were observed among fish fed the diets with equal to or higher than 49.0 mg/kg of vitamin E. The serum lysozyme activity and total complement activity of the fish fed with the control diet were significantly lower than those with vitamin E supplemented diets, the serum lysozyme activity and total complement activity of Japanese seabass increased significantly with increasing dietary vitamin E supplement, and the Japanese seahass had the maximal level of lysozyme activity and total complement activity when fed 98.8 mg/kg. Based on the

关 键 词：花鲈 维生素E 生长 组织中维生素E积累量 免疫 

分 类 号：S966.12[农业科学—水产养殖]