扇贝异源四倍体诱导的初步研究  

Primary study on induction of scallop allotetraploid in cross-fertilized eggs of Chlamys farreri♀×Patinopecten yessoensis♂

作　　者：迟长凤[1,2] 杨爱国[1] 王清印[1] 刘志鸿[1] 周丽青[1] 吴常文[2] 

机构地区：[1]中国水产科学研究院黄海水产研究所,山东青岛266071 [2]浙江海洋学院海洋科学学院,浙江舟山316004

出　　处：《水产学报》2009年第1期165-170,共6页Journal of Fisheries of China

基　　金：国家科技支撑计划专题项目(2006BAD01A00);国家“八六三”高技术研究发展计划(2006AA10A408);国家自然科学基金项目(30600465)

摘　　要：The allotetraploid in cross-fertilized eggs of Chlamys farreri♀× Patinopecten yessoensis♂ was induced in this paper.In order to find out the appropriate duration time of 6-DMAP treatment,ploidy status of induction offspring was analyzed by flow cytometry.The results showed that the optimal duration time of 6-DMAP treatment was to treat the fertilized eggs with 50 mg/L 6-DMAP for 15 min based on the integrated evaluation of the results of fertilization rate,survival rate of D-larvae,development status of larvae,and percentage of tetraploid.The results of flow cytometry showed that 17.3% tetraploid was obtained which showed that allotetraploid could be gotten with this method.The causes of the problems in the work were also discussed in this paper.The allotetraploid in cross-fertilized eggs of Chlamys farreri ♀ × Patinopecten yessoensis ♂ was induced in this paper. In order to find out the appropriate duration time of 6-DMAP treatment, ploidy status of induction offspring was analyzed by flow cytometry. The results showed that the optimal duration time of 6-DMAP treatment was to treat the fertilized eggs with 50 mg/L 6-DMAP for 15 rain based on the integrated evaluation of the results of fertilization rate, survival rate of D-larvae, development status of larvae, and percentage of tetraploid. The results of flow cytometry showed that 17.3% tetraploid was obtained which showed that allotetraploid could be gotten with this method. The causes of the problems in the work were also discussed in this paper.

关 键 词：栉孔扇贝 虾夷扇贝 异源四倍体 流式细胞术 

分 类 号：S917[农业科学—水产科学]