荧光偏振法用于止血剂与脂质体及血液红细胞主-客体分子间作用机制的研究  

HOSTGUEST MOLECULE INTERACTION MECHANISM OF HEMOSTATICS WITH LIPOSOMES AND RED BLOOD CELLS STUDIED WITH FLUORESCENCE POLARIMETRIC METHOD

作　　者：杜鸣[1,2,3] 唐波[1,2,3] 沈含熙[1,2,3] 汪树玉 孙天麟[1,2,3] 张超 

机构地区：[1]山东师范大学化学系 [2]南开大学化学系 [3]山东省产品质量监督检验所

出　　处：《药学学报》1998年第5期362-368,共7页Acta Pharmaceutica Sinica

基　　金：国家博士点基金;山东省青年自然科学基金

摘　　要：以磷脂脂质体模拟血液细胞，用１，６二苯基已三烯（ＤＰＨ）为荧光探针，荧光偏振法研究了安络血、止血芳酸、维生素Ｋ１和６氨基已酸４种止血剂作为客体分子与脂质体及血液红细胞两类主体分子互相作用而形成的超分子化合物。利用偏振度（Ｐ）与微粘度（η）的定量关系，计算微粘度的变化，并以微粘度的变化推测止血剂与红细胞的作用机制。结果表明，止血剂与脂质体或血红细胞的相互作用力主要是超分子作用力。不同血凝机制的止血剂与红细胞作用方式也不尽相同。本文还对止血剂与红细胞的结合方式进行了探讨。The supermolecule compounds of adrenobazone, paminomethylbenzoic acid, vitamin K1, 6amino caproic acid with liposomes and red blood cells were studied by fluorescence polarimetric method. The mechanisms of formation of the supermolecule compounds were examined by fluorescence probe of the link of 1,6dipheny1,3,5hexatriene (DPH) with liposomes which were taken as a model of blood cells. The interaction mechanism of hemostatics with red blood cells was described according the quantitative relationship between polarization value (P) and the microviscosity (=2P046-P). The result showed that the acting force between hemostatics and liposomes or that between hemostatics and red blood cells were mainly supermolecular acting force. The acting force between vitamin K1 and cytomembrane is hydrophobic force and those between adrenobazone, paminomethylbenzoic acid, or 6amino caproic acid and cytomembrane are hydrogen bond or electrostatic force. Under the same drug concetration, all of the four haemostatics can reduce the fluidity of the cell membrane, which benefits blood coagulation. The binding ways of hemostatics with red blood cells was also discussed.

关 键 词：荧光偏振 止血剂 脂质体 血液红细胞 药理学 

分 类 号：R973.1[医药卫生—药品] R944.9[医药卫生—药学]