盐酸诺氟沙星在奥尼罗非鱼体内的残留消除规律  

Residue Elimination of Norfloxacin Hydrochloride in Hybrid Tilapia(Oreochromis aureus×O.niloticus)

作　　者：范祯艳[1] 张其中[1,3] 陈国权[2] 甘安辉 于相满[1] 朱泽尧[1] 

机构地区：[1]暨南大学水生生物研究所,广州510632 [2]广州市白云区农业技术推广中心,广州510405 [3]水产科学重庆市市级重点实验室,三峡库区生态环境教育部重点实验室,重庆400715

出　　处：《淡水渔业》2008年第6期55-58,共4页Freshwater Fisheries

基　　金：广州市白云区科技计划项目(2006-sz-20)

摘　　要：用高效液相色谱法（HPLC）研究盐酸诺氟沙星在奥尼罗非鱼（Oreochromis aureus×O.niloticus）体内残留消除规律。结果显示：此方法回收率为73.08%~89.61%,最低检测限为0.2 ng/mL;灌药第5天,4种组织药物含量达到最高,随后迅速下降;药物残留消除速率为：血液〉肌肉〉肝脏〉肾脏。结果表明：在（23±1）℃水温条件下,盐酸诺氟沙星在罗非鱼肌肉中降到50μg/kg的休药期为13 d,在罗非鱼内脏中降到50μg/kg的休药期为22 d。Tissue residue elimination of norfloxacin hydrochloride was evaluated in hybrid tilapia （ Oreochromis aureus ×O. niloticus）. Norfloxacin hydrochloride concentrations were determined by means of high performance liquid chromatography（HPLC） with 0. 2 ng/mL detection limit, and the overall recoveries were 73.08% -89. 61%. Results of this study showed that norfloxacin hydrochloride residues gradually accumulated in four tissues during dosing time and the highest level was observed on the 5^th dosing day（ the last dosing time）. The elimination rates were different in the four tissues and the order was serum 〉 muscle 〉 liver 〉 kidney. According to the maximum residues limit（MRL） of 50 μg/kg body weight in edible tissue or viscera, the withdrawal time should be at least 13 days or 22 days prior to the use of dosed tilapia as human food following multi-oral administrations at water temperature of（ 23 ± 1 ） ℃.

关 键 词：盐酸诺氟沙星 残留 奥尼罗非鱼(Oreochromis aureus×O.niloticus) 高效液相色谱法 

分 类 号：S965.125[农业科学—水产养殖] S948[农业科学—水产科学]