冷休克诱导泥鳅三倍体的研究  

Induction of Triploid Mud Loach(Misgurnus anguillicaudatus) by Cold Shock

作　　者：康绍乐[1] 职金华[1] 杜启艳[1] 常重杰[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007

出　　处：《淡水渔业》2008年第6期76-79,共4页Freshwater Fisheries

基　　金：国家自然科学基金(No.30771666);河南省杰出青年科学基金(No.0512001800);河南省省级重点学科动物学资助

摘　　要：采用冷休克抑制第二极体排出的方法研究了泥鳅（Misgurnus anguillicaudatus）三倍体,并探讨了诱导泥鳅三倍体形成的最佳条件。结果显示：受精后3-5 min,用2℃冷水休克处理30 min,不仅可以得到较高的三倍化率,还可维持相当高的成活率。另外,本实验发现泥鳅间期细胞核Ag-NORs的最高数目与染色体组数目具有明显的对应关系,可以作为判断胚胎倍性的简单标准。The study was made to optimize cold shock conditions for producing triploid mud loach（ Misgurnus anguillicaudatus） by inducing second polar body extrusion, h could give relatively higher rate of triptoidy and embryos survival to treat the fertilized eggs for 30 minutes in 2 ℃ water 3 - 5 minutes ＇after fertilization. The results showed that cold shock was an effective method to induce triploidy fish. Moreover, it suggested that the highest number of Ag-NORs in interphase nucleus was correspondent to the nmnber of chromosome set, and served as an indicator for determining the ploidy of embryo. This method is rapid and reliable.

关 键 词：三倍体诱导 冷休克 泥鳅(Misgurnus anguillicaudatus) AG-NOR 

分 类 号：S966.4[农业科学—水产养殖] Q789[农业科学—水产科学]