卵黄抗体用于血吸虫循环抗原检测的可行性研究  

Study on detection of circulating schistosome antigen with egg yolk antibody

作　　者：吴玉龙[1] 张炜明[2] 刘文琪[3] 仇镇宁[2] 冯振卿[2] 李雍龙[3] 管晓虹[2] 

机构地区：[1]滨州医学院病原生物学教研室,滨州市256603 [2]南京医科大学卫生部抗体技术重点实验室 [3]华中科技大学同济医学院人体寄生虫学教研室

出　　处：《滨州医学院学报》2008年第6期401-403,共3页Journal of Binzhou Medical University

基　　金：国家高技术研究发展计划("863"计划;2006AA02Z415);滨州医学院科技计划(BY2006KJ01)

摘　　要：目的探讨抗可溶性虫卵抗原(SEA)鸡卵黄抗体(Immunoglobulin Y,IgY)用于血吸虫循环抗原检测的可行性。方法以纯化的鸡抗SEA卵黄抗体为捕捉抗体,以酶标抗SEA单克隆抗体NP28-5B进行双抗体夹心酶联免疫吸附试验法(S-ELISA)检测急、慢性血吸虫病病人和健康人血清,并与常规检测抗体的酶联免疫吸附试验(SEA-ELISA)法做比较。结果S-ELISA法测得SEA浓度(y)与A450值(x)呈明显的正相关(r=0.9481,y=459.22x-108.14);S-ELISA法检测急性血吸虫病人循环抗原的阳性率为100%,慢性血吸虫病人循环抗原的阳性率为84.4%,健康人的特异性为96.0%;两种方法对血吸虫病的检出率差异无统计学意义(P>0.05)。结论S-ELISA可用于血吸虫病的免疫诊断。Objective To analyze the feasibility on detection of circulating sehistosome antigen with anti-SEA chicken immunoglobulin Y （IgY ）. Methods Using chicken anti-SEA IgY as the capture-antibody and an anti-SEA mouse mAb conjugated with HRP as the detection antibody, a sandwich ELISA technique was established to detect the circulating sehistosome antigen in serum samples of patients with acute, chronic schistosomiasis and healthy people. The results were compared with those of SEA-ELISA. Results There was an obviously positive correlation between SEA concentration and A450 value. The regression equation was deduced （r = 0. 9481 ,y = 459.22x - 108.14 ）. The positive rates of S-ELISA for testing acute, chronic schistosomiasis patients were 100% and 84.4% , respectively. The specificity of healthy people was 96.0%. No significant difference was observed between the two ELISA methods. Conclusion S-ELISA could be used in immunodiagnosis of schistosomiasis.

关 键 词：血吸虫 免疫诊断 循环抗原 卵黄抗体 

分 类 号：R383.24[医药卫生—医学寄生虫学]