促愈瘢痕消皮肤局部用药对动物毒性作用的实验研究  

Animal skin toxic effect experimental study of Cuyubanhenxiao after skin topical administration

作　　者：闫宏山[1] 崔光怀[1] 刘振中[2] 赵建平[2] 杨加峰[2] 蔡景龙[2] 

机构地区：[1]滨州医学院附属医院烧伤美容整形外科,滨州市256603 [2]山东大学第二医院美容整形烧伤外科

出　　处：《滨州医学院学报》2008年第6期419-421,共3页Journal of Binzhou Medical University

基　　金：山东省科技攻关项目(2007GG2NS02055)

摘　　要：目的探讨促愈瘢痕消(70%)促愈瘢痕消对动物皮肤的毒理作用。方法40只新西兰大白兔,随机分为3组。①组12只兔子随机分为A、B、C 3组,每组4只,分别为赋形剂组、促愈瘢痕消临床用药剂量组(44%)、促愈瘢痕消大剂量组(70%)。进行双侧背部脱毛,左侧刀片搔刮至均匀出血,右侧用药7 d观察药物经完整皮肤的急性毒性作用;左侧用药7 d观察药物经损伤皮肤急性毒性作用。②组4只兔子每只背部对称脱毛各2块,药物分组同上,左侧均涂赋形剂,右侧头侧脱毛区涂促愈瘢痕消(44%),右侧尾侧区涂促愈瘢痕消(70%)。观察用药7 d后对完整与损伤皮肤刺激性。③组24只兔子随机分为A、B、C 3组,每组8只,分别为赋形剂组、促愈瘢痕消(44%)组、阳性对照组(1%2,4-二硝基氯代苯)组,进行左侧背部脱毛,各组外用药物进行初次接触及激发试验,观察药物对动物皮肤的过敏反应。结果促愈瘢痕消经皮肤对动物完整皮肤及损伤皮肤无急性毒性、刺激性;药物对完整皮肤亦无激发过敏反应。结论促愈瘢痕消短期应用对动物皮肤是安全的。Objective To study the acute toxicity, irritation and sensitivity effect of Cuyubanhenxiao to New Zealand big white rabbits nomal skin and impaired skin. Methods Fourty New Zealand big white rabbits were dividesed into 3 groups. （1）12 rabbits were randomized into 3 groups, each group is 4 rabbits. The medicine is Vaseline,Cuyubanhenxiao（44% ） ,Cuyubanhenxiao（70% ）. They were separately applied to A,B,C 3 groups. Each rabbit with 50 cm^2 was molted on both sides of backbone to take the acute skin toxicity test. The right sides every group were used medicine（A,B,C） at 7 days, the nomal skin acute toxic effect was observed. The left sides were scratched to even hemorrhage and spreaded medicine on every group rabbit. At 7 days, the damaged skin toxic effect was observed. （2）4 rabbits were molted on both sides of backbone of every rabbit and there were 2 molted areas on each side of every rabbit. The medicine group was completely same with the first group. The left side of every molted area was spreaded by Vaseline. The right molted areas of near to the head were spreaded by B and the oher areas were spreaded by C 7 days later, the nomal and damaged skin irritation effect was observed. （3）24 rabbits were randomized into A, B,and C 3 groups, the number of each group was 8. The medicine was Vaseline, Cuyubanhenxiao（44% ）, 1%2,4-Dinitrochlorobenzol. Each rabbit with 50 cm^2 was molted on the left sides of backbone to take the the initial contact and the stimulation test. The sensitivity effect of medicine was observed. Results There were no acute toxicity, irritation effect, when Cuyubanhenxiao was spreaded on the rabbits＇normal and damaged skin and no sensitivity effect phenomenon to rabbits＇nomal skin. Conclusion It＇s safe to appl to the skin of animals for short-term.

关 键 词：促愈瘢痕消 经皮肤急性毒性 皮肤刺激性 皮肤过敏反应 

分 类 号：R622[医药卫生—整形外科]