联合应用α-半乳糖苷酶和α-N-乙酰半乳糖胺酶实现红细胞AB→O血型转变  

Human RBCs blood group conversion from AB to O by the combination of α-galactosidase and α-N-acetylgalactosaminidase

作　　者：徐华[1,2] 郁成雨[1] 章扬培[1] 

机构地区：[1]军事医学科学院野战输血研究所血液分子生物学研究室,北京100850 [2]陕西省血液中心

出　　处：《中国输血杂志》2008年第12期917-920,共4页Chinese Journal of Blood Transfusion

基　　金：国家高技术研究发展计划(863项目)(批准号:102-09-04-02);国家重点基础研究发展规划(973项目)(批准号:2002CB713800);全军"十五"重点课题(批准号:2000252910)资助项目

摘　　要：目的研究酶解去除AB型红细胞表面的A和B抗原、实现AB→O血型转变的方法,以获得通用型红细胞。方法联合应用α-半乳糖苷酶和α-N-乙酰半乳糖胺酶酶解AB型红细胞,用单克隆抗-A、抗-A1和抗-B抗体检测酶解效果,用稀有血型抗体检测酶解前后红细胞表面的稀有血型,用流式细胞术检测酶解前后红细胞表面A、B、H抗原,原子力显微镜观察酶解前后红细胞形态,主侧配血试验检测酶解红细胞是否符合临床输血要求。结果A1B和A2B亚型红细胞的A、A1和B抗原均能够完全被酶解去除,酶解前后红细胞的稀有血型抗原不变;流式细胞术显示,酶解后AB型红细胞表面的A和B抗原消失,而H抗原明显增加,和O型红细胞相当;酶解不影响红细胞的形态;主侧配血试验证明酶解改造后的AB型红细胞可以安全输给O、A、B、AB等各型受血者。结论联合应用α-半乳糖苷酶和α-N-乙酰半乳糖胺酶成功实现了AB→O血型转变,获得酶解通用型红细胞。Objective To realize RBCs blood group conversion by cleaving A and B antigens with α-galactosidase and α-N-aeetylgalactosaminidase. Methods Human RBCs of blood group AB were treated with α-galactosidase and α-N- acetylgalactosaminidase. Enzyme-converted RBCs were typed using monoclonal anti-A, anti-A1 and anti-B. The main rare blood groups were tested using the antibodies. The antigens of A/B/H were detected by FCM. The shape of RBCs was observed by atomic force microscope. The main side cross-match tests of enzyme-converted RBCs were also conducted. Resuits The A, A1 and B antigens were cleaved completely by the combination of α-galactosidase and α-N-acetylgalactosaminidase. And after the enzymatic treatment, the main rare blood groups hadnt changed. FCM showed that the quantity of A, B antigens and H antigens of enzyme-converted group AB RBCs was as many as that of native group O RBCs. The shape of enzyme-converted group AB RBCs hadn＇t changed. The main side cross-match tests suggested that the enzyme-converted group AB RBCs could he safely transfused to recipients with group O, A, B or AB. Conclusion Human RBCs group could be converted from AB to O by the combination α-galaetosidase and α-N-acetylgalactosaminidase. The universal RBCs could be obtained using glycosidases.

关 键 词：Α-半乳糖苷酶 α-N-乙酰半乳糖胺酶 血型转变 通用型红细胞 

分 类 号：R457.11[医药卫生—治疗学]