超高效液相色谱/串联质谱分析生活饮用水中2,4-滴、2,4,6-三氯酚和五氯酚的方法研究  

Determination of 2,4-D、2,4,6-trichlorophenol and pentachlorophenol in drinking water by UPLC-MS/MS

作　　者：陈砚朦[1] 钟淑婷[1] 甘凤娟[1] 韩媛媛[1] 肖湖[1] 周明灿[1] 张少彬[1] 

机构地区：[1]广东省东莞市疾病预防控制中心,广东东莞523000

出　　处：《中国卫生检验杂志》2008年第12期2463-2466,共4页Chinese Journal of Health Laboratory Technology

摘　　要：目的：建立检测生活饮用水中2,4-滴、2,4,6-三氯酚和五氯酚的超高效液相色谱/串联质谱法（UPLC-MS/MS）。方法：水样经高速离心,Waters ACQUITY UPLC BEHC181.0×50 mm柱分离,以纯水和乙腈为流动相,采用UPLC-ESI-MS/MS法,以负离子检测方式,多离子反应监测（MRM）定量法检测生活饮用水中2,4-滴、2,4,6-三氯酚和五氯酚。结果：经方法学验证,该方法对2,4-滴、2,4,6-三氯酚和五氯酚的最低检测限分别为0.5、3.0、1.0μg/L（进样量10μl）。在线性范围中,相关系数r均大于0.99,平均回收率分别在85%～115%之间。结论：方法快速、灵敏度高、操作简单、定量准确、测定浓度范围宽阔,是生活饮用水样品中2,4-滴、2,4,6-三氯酚和五氯酚含量快速检测的理想方法。对东莞市生活饮用水样品检测表明：此水体中2,4-滴、2,4,6-三氯酚和五氯酚的含量都低于检测限。Objective：A UPLC-MS/MS method was developed for the determination of 2,4-D,2,4,6-trichlorophenol and Pentachlorophenol in drinking water.Methods：After centrifugation,determined by UPLC-ESI（-）-MS/MS,quantified with multiple reaction monitoring（MRM） mode,performed on a Waters ACQUITY UPLC BEH C18 1.0×50 mm column,water and acetonitrile was used as mobile phase.Results：The detection limits（LOD） for2,4-D,2,4,6-trichlorophenol and pentachlorophenol were 0.5,3.0,1.0 μg/L.In the linear ranges,correlation coefficients was above 0.99.The mean recoveries were between 85% and 115%.Conclusion：By coupling the ACQUITY UPLC with Quattro PremierTMXE,a rapid and sensitive method has been developed for the trace level quantitation and confirmation of 2,4-D,2,4,6-trichlorophenol and PCP in drinking water for its excellent sensitivity and selectivity.

关 键 词：超高效液相色谱-电喷雾串联四极杆质谱 多离子反应监测(MRM) 2 4-滴 2 4 6-三氯酚 五氯酚 

分 类 号：O657.72[理学—分析化学]