一株好氧反硝化细菌的分离与鉴定试证  

Isolation and identification for an aerobic strain of denitrobacteria

作　　者：苏俊峰[1,2] 马放[1] 高珊珊[1] 候宁[1] 王弘宇[1] 王强[1] 

机构地区：[1]哈尔滨工业大学市政环境工程学院,哈尔滨150090 [2]西安建筑科技大学环境与市政工程学院,西安710055

出　　处：《哈尔滨工业大学学报》2008年第12期1919-1922,共4页Journal of Harbin Institute of Technology

基　　金：国家自然科学基金重大国际合作项目(50521140075);国家重点基础研究发展计划项目(973-2004CB185050)

摘　　要：为筛选好氧反硝化细菌,采用SBR反应器,通过间歇曝气方式对活性污泥体系中好氧反硝化细菌进行选择和富集,从污泥中筛选出一株好氧反硝化细菌f4,该菌株在完全好氧的条件下可高效地将NO3-N反硝化为N2,对NO3-N去除率达到90%以上,并不产生NO2-N的累积,反硝化过程或氮气生成过程都发生在对数生长期,其培养特征为ORP降低和反硝化产碱.经过生理生化鉴定和16SDNA测序,建立系统发育树,可基本确定分离的菌株f4Pseudomonas.sp.To isolate the aerobic strain of denitrifiers, the method of intermittent aeration was adopted to select and enrich aerobic denitrifiers by domesticating the activated sludge with an SBR. Aerobic denitrifiers f4 were isolated from it. The strain has good performance of denitrification in absolute aerobic environment and there is no NO2 - N - accumulation, meanwhile, the removal rate of NO3 - N can reach over 90 %. The denitrifyication process can be described as NO3 - N→NO2 - N→N2, and it mainly occurs in Log Phase with the increase of pH and the decrease of ORP. According to the physiological biochemical index, strains f4 of the aerobic denitrifier is generally identified as Pseudomonas. sp.

关 键 词：好氧反硝化菌 16sDNA 好氧反硝化 分离鉴定 

分 类 号：TU991.2[建筑科学—市政工程]