大气O_3浓度升高对麦田土壤重金属铜生物有效性和生理毒性的影响  

Effect of Elevated Atmospheric Ozone Concentration on the Bioavailability of Cu in Soil and Oxidative Stress of Cu in Wheat(Triticum aestivum L. cv. Yangmai14)

作　　者：周辉[1] 朱建国[2] 孙媛媛[3] 尹颖[1] 王晓蓉[1] 郭红岩[1,2] 

机构地区：[1]污染控制与资源化研究国家重点实验室,南京大学环境学院,江苏南京210093 [2]中国科学院南京土壤研究所,土壤与农业可持续发展国家重点实验室,江苏南京210008 [3]污染控制与资源化研究国家重点实验室,南京大学水科学系,江苏南京210093

出　　处：《农业环境科学学报》2009年第1期21-25,共5页Journal of Agro-Environment Science

基　　金：国家自然科学基金项目(20777034;40110817);中科院知识创新项目(KZCX3-SW-440);江苏省博士后科研资助计划项目

摘　　要：在开放式空气O3浓度增加(FACE)平台下,采用盆栽试验,初步研究了O3浓度升高后麦田重金属Cu的生物有效性变化以及对各生长阶段小麦叶片生理毒性的影响。结果表明,在FACE条件下,小麦地上部对Cu的吸收相比于正常大气对应组有增加的现象;与正常大气对应组相比,FACE条件下土壤中有效态Cu的含量也有所增加;随着小麦的生长发育,FACE圈小麦叶片内的MDA含量总体呈上升趋势,O3升高铜污染组的小麦叶片内MDA的含量最高;与正常大气对照组相比,O3升高铜污染组的小麦叶片SOD酶和POD酶比较敏感,在分蘖期其活性受到诱导,但随着暴露时间的增加,抗氧化系统的各个酶的活性逐渐受到抑制。O3加剧了Cu对小麦的生理胁迫,增加了Cu的生物有效性。Using a Free Air Concentration Enrichment （FACE）system, we studied the effects of elevated O3 concentrations on bioaccumu- lation of Cu in wheat plant and its oxidative stress to wheat plant in pot. At elevated O3 concentration, both concentration of available Cu in soil and uptake of Cu by wheat shoots increased. The malondialdehyde（ MDA ）contents of wheat plants grown in Cu-contaminated soil at elevated O3 concentrations were significantly higher than the control. At the wheat mid-tillering growth stage, we observed an increased activity of superoxIde dismutase （SOD, EC 1.12.1.1 ）and phenol peroxidase（POD, EC 1.11.1.7 ）in the Cu and elevated O3 treatments. However, activities of these antioxidant enzymes were induced by Cu and elevated O3 treatments at the wheat panicle initiation growth stage, and inhibited over the exposure time. It can be concluded that elevated O3 concentration can enhance Cu bioavailability and toxicity to wheat, which results in increased oxidative stresses and damage to the self-protection mechanisms of wheat plant.

关 键 词：O3浓度升高 小麦 土壤铜污染 SOD CAT POD APX 

分 类 号：Q945.78[生物学—植物学]