Tb(Ⅲ)对不同生长阶段辣根过氧化物酶生态毒性:光照效应  

The Ecotoxicity Effect of Tb(Ⅲ)on Horseradish POD During Different Growing Periods:Light Intensity

作　　者：张颂婧[1] 周青[1,2] 

机构地区：[1]江南大学工业生物技术教育部重点实验室,江苏无锡214122 [2]江南大学环境与土木工程学院,江苏无锡214122

出　　处：《农业环境科学学报》2009年第1期49-53,共5页Journal of Agro-Environment Science

基　　金：国家自然科学基金(30570323);国家发改委稀土国际合作项目(IFZ051210)

摘　　要：实验以辣根为研究材料,采用生态毒理学中的急性毒性试验方法研究不同生长阶段与不同光照强度条件下Tb(Ⅲ)对辣根过氧化物酶(HRP)的生态毒性。结果表明,比较不同生长阶段不同光照强度下对照CK的HRP活性,数据显示不同生长阶段HRP活性对光强响应不尽一致。辣根在不同生长阶段和不同光照强度下,HRP对高、低剂量Tb(Ⅲ)毒害的响应存在明显差异。进一步比较不同光照强度各生长阶段的半效应剂量(EC50)及Tb(Ⅲ)对HRP酶活的最小作用剂量(LOEC),最大无作用剂量(NOEC)变化规律,不同生长阶段光照强度对Tb(Ⅲ)的生态毒性的影响不同,9、11月份Tb(Ⅲ)的生态毒性受光强变化影响较小,但9月份Tb(Ⅲ)对HRP的毒害作用最小,11月份最大;5月份HRP对强光敏感,7、9月份HRP对强、弱光均较敏感,11月份HRP光敏性较差,各阶段中强光下HRP对Tb(Ⅲ)胁迫的敏感性小于强、弱光。The environmental safe of rare earth used in agriculture is the key problem to limit rare earth application on agriculture. Horseradish was used as an experimental material to study ecotoxicity of Terbium at different light intensities during different growing periods by using the method of determining acute toxicity. The results showed that effects of light intensity on the activities of Horseradish POD （HRP） were different in different growing periods by comparison of the activities of HRP of control （CK）. And there was a significant difference between the response of HRP to high and low concentration of Tb（ Ⅲ ）under different light intensities during different growing periods. The results also indicated that the effects of light intensity on Terbium ecotoxicity had a remarkable difference in different growing periods by the comparison of 24 h-EC50 of Tb（Ⅲ ）and LOEC and NOEC. Light intensity had little effect on the ecotoxicity of Terbium in September and November, but the ecotoxicity of Terbium on HRP was the least（most）remarkable in September（November）. Among all the growing periods May was sensitive to weak light while July and September were sensitive to both strong and weak light, November was less sensitive, but all periods were less sensitive to natural light intensity.

关 键 词：生态毒理学 辣根 铽 HRP 生长阶段 光照强度 

分 类 号：Q945.12[生物学—植物学]