金边瑞香浸膏体内外抗肿瘤作用  

Antitumor effect of extract from Daphne odora Var. Marginata in vivo and in vitro

作　　者：徐静[1,2] 傅颖媛[1] 黄彬红[2] 曾小平[1] 况南珍[1] 周智兴[1] 

机构地区：[1]南昌大学医学院免疫学教研室,南昌330006 [2]赣南医学院生化与分子生物学教研室,赣州341000

出　　处：《中国新药杂志》2008年第23期2019-2021,2026,共4页Chinese Journal of New Drugs

基　　金：江西省卫生厅中医药科研基金(04A15)

摘　　要：目的：探讨金边瑞香浸膏的体内外抗肿瘤作用。方法：采用流式细胞术观察不同浓度金边瑞香浸膏对SMMC7721细胞周期的影响。采用MTT法检测不同浓度金边瑞香浸膏对SMMC7721，NKM45，A549和S180肿瘤细胞增殖的影响。利用小鼠皮下移植性肿瘤模型观察不同浓度金边瑞香浸膏的体内抗肿瘤作用。结果：金边瑞香浸膏可使SMMC7721 G0／C1期细胞增多，S期细胞减少，以0．5g·mL^-1剂量作用最明显，G0／G1期细胞达80．64％。金边瑞香浸膏浓度在0．03125～1．0g·mL^-1范围内对体外培养的SMMC7721，NKM45，A549和S180肿瘤细胞增殖均有一定程度的抑制作用，0．5g·mL^-1浓度时对此4种肿瘤细胞的增殖抑制率分别为65．82％，60．38％，42．36％和49．94％。金边瑞香浸膏对荷S180小鼠的肿瘤生长有抑制作用，20和40g·kg^-1·d^-1金边瑞香剂量组抑瘤率分别为43．57％和28．13％。结论：金边瑞香浸膏在体内外具有抗肿瘤作用。Objective： To investigate the antitumor effect of extract from Daphne odora Var. Marginata in vivo and in vitro, nethods：SMMC7721 cells were cultured in the absence or presence of extract from Daphne odora Var. Marginata. Cell cycle was determined by flow cytometry. The effect of the extract on growth of SMMC7721, NKM45, A549 and S180 cancer cell lines was evaluated by MTT reduction assay. The in vivo anti-tumor effect was examined in tumor-bearing mice. Results： The extract increased the ratio of G0/G1 phase in SMMC7721 cells, and decreased the proportion of S phase cells. The ratio of G0/G1 phase cells was 80.64% after exposure to 0.5 g·mL^-1 of the extract. The extract （0. 031 25 - 1.0 g · mL^-1 ） concentration-dependently inhibited growth of SMMC7721, NKM45, A549 and S180 ceils. The inhibition rate at 0.5 g·mL^-1 was 65.82% , 60.38% , 42.36% and 49.94% for SMMC7721, NKM45, A549 and S180 cells, respectively. The extract reduced tumor weigh by 43.57% and 28.13% in vivo at 20 and 40 g· kg^-1· d^-1 in S180-tummor-bearing mice. Conclusion： Extract from Daphne odora Var. Marginata possesses the antitumor effect in vivo and in vitro.

关 键 词：金边瑞香 抗肿瘤作用 细胞周期 MTT法 

分 类 号：R286.91[医药卫生—中药学]