基于金纳米颗粒免疫凝集的压电传感技术快速检测人血清免疫球蛋白IgG  

A piezoelectric immunoagglutination assay for IgG using gold nanoparticles

作　　者：刘佳[1] 丁艳君[1] 沈国励[1] 俞汝勤[1] 

机构地区：[1]湖南大学化学化工学院,化学/生物传感与计量学国家重点实验室,湖南长沙410082

出　　处：《化学传感器》2008年第4期31-35,共5页Chemical Sensors

基　　金：国家自然科学基金(批准号:20435010;20375012)

摘　　要：发展了一种利用金纳米颗粒免疫凝集的压电传感技术用于人血清免疫球蛋白IgG的简单、快速、高灵敏检测。以金纳米颗粒替代传统胶乳标记羊抗人IgG诊断血清(抗IgG),利用石英晶体微天平(QCM)直接灵敏响应因金纳米颗粒免疫凝集而引起溶液的非质量参数(密度、粘度等)的变化。考察了pH值、离子强度和抗IgG-金纳米颗粒浓度对免疫凝集反应的影响,并进行了质控实验。结果表明,该传感技术毋需固定活性组分,可快速检测浓度下限0.38μg/mL的免疫球蛋白IgG。定量能力与经典ELISA法相接近,可基本满足临床疾病诊断的生化检测要求。A simple, rapid, and highly sensitive agglutination - based piezoelectric immunoassay has been developed for directly detecting IgG immunoglobulin in human serum. The proposed technique is based on the specific agglutination of antibody - coated gold nanoparticles in the presence of the corresponding antigen, which causes a frequency change that can be monitored by a piezoelectric quartz crystal microbalance （QCM）. In contrast to the commonly used piezoelectric methods, it possesses an attractive advantage in that the immobilization of antibody or antigen on the crystal surface is unnecessary. It is found that the developed immunoagglutination assay system is sensitive to concentration of IgG as low as 0. 38 ~g/mL. Analytical results of several specimens obtained using the developed technique are in satisfactory agreement with those given by the ELISA method. Implying a promising alternative approach for detection IgG in the clinical diagnosis.

关 键 词：金纳米颗粒 石英晶体微天平 免疫凝集 

分 类 号：R446.6[医药卫生—诊断学]