AChE/PAMAM-Au/CNTs/GC传感器用于有机磷农药检测的研究  

AChE/PAMAM-Au/CNTs/GC biosensor application on determination of organophosphorus pesticide

作　　者：肖飞[1] 曲云鹤[1] 卫银银[1] 金利通[1] 

机构地区：[1]华东师范大学化学系,上海200062

出　　处：《化学传感器》2008年第4期36-41,共6页Chemical Sensors

基　　金：上海市科委No.06dz05824资助项目

摘　　要：该文制备了一种多层AChE/PAMAM-Au/CNTs/GC乙酰胆碱酯酶的酶抑制电流型传感器应用于有机磷农药的检测,主要利用碳纳米管良好的导电性和吸附性,以及PAMAM(G4)-Au树枝状复合物特殊的结构及导电性能,利用有机磷农药对乙酰胆碱酯酶的抑制作用,以硫代乙酰胆碱(ATCh)为底物,实现了对有机磷农药的检测。实验表明,该方法快速简单,线性范围宽,灵敏度高。固定在传感器上的乙酰胆碱酯酶具有良好的酶动力学响应,其米氏常数(KMapp)为1.66mmol/L。对有机磷农药呋喃丹的最低检测限达到4.0nmol/L。In this study, PAMAM- Au/CNTs/GC electrode was prepared used for the fabrication of acetyl- cholinesterase （ACHE）immobilized electrochemieal sensor for the detection of organophosphorus pestieides （OPs）, such as carbofuran. The degree of inhibition of the AChE enzyme by OPs compounds was determined by measuring the oxidation current of the thiocholine generated by the AChE catalyzed hydrolysis of acetyhhiocholine （ATCh） . It was shown that the method of offered a fast, sensitive and stable response. A value of 1.66 mmol/L for the apparent Michaelis - Menten constant （ KM^app） was obtained. The detection limit of the biosensor is 4. 0 nmol/L.

关 键 词：AChE/PAMAM—Au/CNTs/GC 乙酰胆碱酯酶 有机磷农药 

分 类 号：TP212.9[自动化与计算机技术—检测技术与自动化装置]