HPLC测定清金得生片华蟾酥毒基和酯蟾毒配基含量  

Determination of Cinobufagin and Recibufogenin in Qingjindesheng Tablets by HPLC

作　　者：陈木水[1] 黄月纯[1] 唐洪梅[1] 曾惠芳[1] 蔡庆群[1] 

机构地区：[1]广州中医药大学第一附属医院,广州510405

出　　处：《中国药师》2008年第12期1487-1488,共2页China Pharmacist

摘　　要：目的:建立清金得生片中华蟾酥毒基和酯蟾毒配基的含量测定方法。方法:采用Hypersil ODS色谱柱(250mm×4mm,5μm);以乙腈-0.5%磷酸二氢钾溶液(用磷酸调pH至3.2)(45:55)为流动相;检测波长为296nm;柱温35℃。结果:华蟾酥毒基和酯蟾毒配基分别在0.04～1.25μg(r=0.999 9)、0.04～1.24μg(r=0.9998)范围内线性关系良好,加样回收率分别为99.37%(RSD=1.71%),100.20%(RSD=1.37%)。结论:方法简便、结果准确。Objective： To establish a method for determination the effective components in Qingjindesheng tablets. Method： The Hypersil ODS column（250mm × 4mm, 5 μm ） was used, the mobile phase was acetonitrile -0.5% potassium dihydrogen phosphate （ ajust pH to 3.2 with phosphonic acid） （45：55 ）. The detective wavelength was at 296 nm. The column temperature was 35 ℃. Result： Cinobufagin was in good linearity between 0.04μg and 1.25μg（ r = 0. 999 9 ） , and the average recovery was 99.37% , and RSD was 1.71% ; Recibufogenin was in good linearity between 0.04μg and 1.24μg（ r = 0.999 8 ） , and the average recovery was 100.20% , and RSD was 1.37%. Conclusion： The method is simple, accurate, it can be used as one of the quality control of the Qingjindesheng tablets.

关 键 词：清金得生片 华蟾酥毒基 酯蟾毒配基HPLC 

分 类 号：R927.2[医药卫生—药学]