烟雾吸入伤后大鼠肺及肾血管紧张素转换酶表达变化的研究  

Changes of Angiotensin-Converting Enzyme and Angiotensin-Converting Enzyme 2 in Lung and Kidney of Rats after Smoke Inhalation Injury

作　　者：高维[1] 金发光[1] 李圆园[2] 保鹏涛[3] 

机构地区：[1]第四军医大学唐都医院呼吸科,陕西西安710038 [2]解放军253医院急诊科,内蒙古呼和浩特010051 [3]第四军医大学西京医院呼吸科,陕西西安710032

出　　处：《中国呼吸与危重监护杂志》2009年第1期63-67,共5页Chinese Journal of Respiratory and Critical Care Medicine

基　　金：全军“十五”重点项目(编号:01Z074)

摘　　要：目的探讨血管紧张素转换酶(ACE)和ACE2在烟雾吸入伤后大鼠肺、肾的表达变化和可能的作用机制。方法30只健康雄性SD大鼠随机分为正常对照组,伤后1、4、10和24 h组,每组各6只。致伤组予以浓烟吸入30 min,按组别不同在相应时间点取其肺、肾组织,分别进行肺组织湿/干重测定、HE染色和ACE、ACE2免疫组织化学检测。结果致伤组大鼠均表现出急性肺损伤症状,肺湿/干重均显著高于正常对照组(P<0.05或P<0.01)。ACE、ACE2在正常肺、肾细胞膜上有表达。肺内ACE表达在致伤后立即升高;而肺内ACE2在致伤后4 h开始明显升高。各组间肾组织ACE及ACE2表达无明显差别。结论肺组织ACE、ACE2表达的不同变化在烟雾吸入伤的发生机制中可能有重要作用。Objective To observe the expression of angiotensin-converting enzyme（ACE） and ACE2 in rat lungand kidney at different time point after smoke inhalation injury and to explore the possible mechanism. Methods Thirty healthy male SD rats were randomly divided into five groups （ n = 6 in each group ） , ie, a normal control group,and 4 injury groups of 1 h,4 h, 10 h and 24 h respectively after 30 min of dense smoke inhalation. The rats were sacrificed at different time point. Lung and kidney tissue samples were collected for measurement of lung wet/ dry weight ratio （W/D） , pathological study by HE staining, and ACE and ACE2 expression by immunochemistry staining. Results The inhaled rats all displayed acute lung injury symptoms. The lung W/D in the injury groups were significantly higher compared to the normal control group （ P 〈 0. 05, P 〈 0. 01 ） . ACE and ACE2 were expressed on cellular membrane of normal lung and kidney. The expression of ACE in lung was increased immediately after injury and the expression of ACE2 in lung was increased at 4 h after injury. No significant changes of the expression of ACE and ACE2 in kidney were observed in the five groups. Conclusion The imbalances of expression of ACE and ACE2 in lung might play an important role in the pathogenesis of smoke inhalation injury.

关 键 词：烟雾吸入 急性肺损伤 血管紧张素转换酶 血管紧张素转换酶2 

分 类 号：R563[医药卫生—呼吸系统]