Isolation and phylogenetic analysis of heterotrophic nitrobacteria  

作　　者：苏俊峰 马放 郭静波 侯宁 高珊珊 王弘宇 

机构地区：[1]School of Municipal and Environmental Engineering,Harbin Institute of Technology [2]School of Environmental and Municipal Engineering,Xi'an University of Architecture and Technology [3]Dept.of Municipal Engineering,Wuhan University

出　　处：《Journal of Harbin Institute of Technology(New Series)》2008年第6期790-793,共4页哈尔滨工业大学学报（英文版）

基　　金：Sponsored by the National Natural Science Foundation of China(Grant No.50521140075)

摘　　要：A method about the isolation of heterotrophic nitrosobactria and the characteristics of heterotrophic nitrosobactria were studied.It can be seen from the fluorescence in situ hybridization results of the bio-membrane sample from the bio-ceramic reactor,the spots of green nitrosobacteria are obviously more than those of orange nitrobacteria.Two heterotrophic nitrobacteria were isolated from the bio-ceramic reactor.By sequencing 16SrDNA and establishing the phylogenic tree,they were identified physiologically and biochemically as Pseudomonas sp..After 12 d,the COD removal efficiency of wgy55 and wgy68 were 45.03% and 50.85%,the NH4-N removal efficiency of them were 80.12% and 85.93%,and the TN removal efficiency of them were 69.71% and 64.7%.The final concentration of NO2-N of wgy55 and wgy68 was 0.753 mg/L and 0.601 mg/L,and that of NO3-N was 3.21 mg/L and 3.38 mg/L.These results show that wgy55 and wgy68 have the capability of nitrification and they are heterotrophic nitrobacteria.A method about the isolation of heterotrophic nitrosobactria and the characteristics of heterotrophic nitrosobactria were studied. It can be seen from the fluorescence in situ hybridization results of the bio-membrane sample from the bio-ceramic reactor, the spots of green nitrosobacteria are obviously more than those of orange nitrobacteria. Two heterotrophic nitrobacteria were isolated from the bio-ceramic reactor. By sequencing 16SrDNA and establishing the phylogenic tree, they were identified physiologically and biochemically as Pseudomonas sp.. After 12 d, the COD removal efficiency of wgy55 and wgy68 were 45.03% and 50. 85% , the NH4-N removal efficiency of them were 80. 12% and 85.93% , and the TN removal efficiency of them were 69. 71% and 64. 7%. The final concentration of NO2-N of wgy55 and wgy68 was 0. 753 mg/L and 0. 601 mg/ L, and that of NO3-N was 3.21 mg/L and 3.38 mg/L. These results show that wgy55 and wgy68 have the capability of nitrification and they are heterotrophic nitrobacteria.

关 键 词：bio-ceranfic reactor fluorescence in situ hybridization heterotrophic nitrification 

分 类 号：X703[环境科学与工程—环境工程]