韭莲组培快繁及其石蒜碱和加兰他敏积累的研究  

Studies on Tissue Culture and Rapid Propagation of Zephyranthes grandiflora and Accumulation of Lycorine and Galanthamine

作　　者：冯巍[1,2,3] 谈锋[1,2,3] 谢峻[1] 陈斌[1] 苏静[1] 

机构地区：[1]西南大学生命科学学院,重庆400715 [2]三峡库区生态环境教育部重点实验室,重庆400715 [3]重庆市三峡库区植物生态与资源重点实验室,重庆400715

出　　处：《西南大学学报（自然科学版）》2008年第12期134-138,共5页Journal of Southwest University(Natural Science Edition)

基　　金：重庆市三峡库区植物生态与资源重点实验室资助项目

摘　　要：以韭莲组培苗鳞茎为试验材料,通过正交试验筛选出最佳的小鳞茎增殖培养基:MS+6-BA 0.5 mg/L+NAA 1.5 mg/L+蔗糖50 g/L.将诱导得到的小鳞茎苗转入添加了不同激素和蔗糖浓度的1/2MS培养基上,得到最适生根培养基为1/2MS+NAA 0.5 mg/L+蔗糖15 g/L,移栽成活率100%.利用RP-HPLC法测定组培苗和野生苗鳞茎中石蒜碱和加兰他敏的含量,韭莲组培苗鳞茎中石蒜碱和加兰他敏的含量分别为204.83μg/g,98.7μg/g;野生苗鳞茎中石蒜碱和加兰他敏的含量分别为225.36μg/g,63.72μg/g.组培苗鳞茎中加兰他敏的含量是野生苗鳞茎的1.55倍.The bulbs of Zephyranthes grandiflora were used as explants in tissue culture. According to the results of orthogonal test L9 （3^4 ）, the optimal medium for bulb multiplication was identified to be MS ＋6-BA 0. 5 mg/L＋NAA 1.5 mg/L＋sucrose 50 g/L. The optimum medium for rhizogenesis was 1/2MS ＋NAA 0.5 mg/L＋sucrose 15 g/L, and a survival rate of 100% was achieved after the transplantation of the rooted plantlets. Determination by RP-HPLC showed that the contents of lycorine and galanthamine were 204.83 μg/g and 98.7μg/g for the tissue culture-derived plantlets, respectively, or the content of galanthamine was 1.5g times for wild Z. grandiflora （225.36 μg/g and 63.72 μg/g）.

关 键 词：韭莲 组织培养 正交试验 石蒜碱 加兰他敏 反相高效液相色谱法 

分 类 号：Q983.11[生物学—人类学]