谷氨酸钠、γ-氨基丁酸注入黑质对大鼠尾壳核二价金属离子转运体1和膜铁转运辅助蛋白表达的影响  

EFFECTS OF INTRANIGRAL INJECTION OF GLUTAMATE AND GABA ON THE EXPRESSION OF DIVALENT METAL TRANSPORTER 1 AND HEPHAESTIN IN THE CAUDATE PUTAMEN NUCLEUS OF RATS

作　　者：关鹏[1] 王娜[1,2] 段相林[1] 常彦忠[1] 

机构地区：[1]河北师范大学分子神经生物学与神经药理学研究所,石家庄050016 [2]河北医科大学基础课部,石家庄050091

出　　处：《解剖学报》2008年第6期795-799,共5页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(30570957)

摘　　要：目的探讨谷氨酸、γ-氨基丁酸(GABA)对大鼠尾壳核铁代谢的影响。方法大鼠立体定位后,向大脑黑质分别注射谷氨酸钠(MSG)和GABA,观察大鼠尾壳核铁含量,黑质多巴胺能神经元酪氨酸羟化酶(TH)的变化以及尾壳核的无铁反应元件结构的二价金属离子转运体1(DMT1-IRE)、膜铁转运辅助蛋白(HP)含量的变化。结果与对照组相比,MSG组大鼠尾壳核铁含量显著增加,GABA组与对照组相比没有显著差异;谷氨酸钠组和GABA组大鼠黑质TH免疫阳性细胞平均吸光度(AA)与对照组相比均无显著差异;与对照组相比,谷氨酸钠组大鼠尾壳核DMT1-IRE表达均显著增加,而GABA组DMT1-IRE表达有明显降低;谷氨酸钠组大鼠尾壳核HP表达显著降低,GABA组HP表达显著增高。结论黑质的谷氨酸和GABA可能通过影响尾壳核DMT1-IRE和HP的表达影响纹状体尾壳核的铁代谢。Objective To investigate the effects of intranigral injection of glutamate and gama amino butyric acid （GABA） on iron metabolism in the caudate putamen nucleus of rats. Methods Glutamate and GABA respectively were stereotaxically injected into the substantia nigra of rats. The levels of iron in the caudate putamcn nucleus were detected by spectrophntometer. The levels of tyrosine hydroxylase （TH） in the substantia nigra, divalent metal transporter 1 without iron response element in the 3＇-UTR （DMTI-IRE） and hephaestin （HP） in the caudate putamen nucleus were also detected by immunohistalogical study. Results The levels of iron in the caudate putamen nucleus of the mono sodium glutamate （MSG） group increased dramatically, while the levels of iron in GABA group decreased with no obvious change. Both the expression of TH in the MSG group and GABA group showed no differences when compared to controls. The levels of DMTI-IRE in the caudate putamen nucleus of the MSG group increased dramatically, while the levels of DMTI-IRE in the GABA group decreased dramatically. However, the levels of HP in the caudate putamen nucleus of MSG group decreased dramatically, while the levels of HP in GABA group increased dramatically. Conclusion Glutamate and GABA in the substantia nigra may affect iron metabolism through DMT1-IRE and HP in the caudate putamen nucleus.

关 键 词：谷氨酸 Γ-氨基丁酸 二价金属离子转运体1 膜铁转运辅助蛋白 免疫组织化学 大鼠 

分 类 号：Q74[生物学—分子生物学]