松果体摘除及褪黑素对大鼠胸腺上皮细胞白细胞介素-7表达的影响  

EFFECT OF PINEALECTOMY AND MELATONIN ON IL-7 EXPRESSION OF RAT THYMIC EPITHELIAL CELLS

作　　者：黄云梅[1] 刘卉[2] 魏建恩[2] 林晋[2] 周瑞祥[2] 

机构地区：[1]福建中医学院福建省高校中西医结合基础重点实验室福建中西医结合研究院 [2]福建医科大学人体解剖学与组织胚胎学系神经生物学研究中心,福州350108

出　　处：《解剖学报》2008年第6期901-905,共5页Acta Anatomica Sinica

基　　金：国家自然科学基金(30840049);福建医科大学教授基金(JS07002)资助

摘　　要：目的探讨松果体摘除及褪黑素对大鼠胸腺上皮细胞白细胞介素7(IL-7)表达的影响及其意义。方法1.培养大鼠胸腺上皮细胞,应用广谱角蛋白抗体进行免疫细胞化学显色鉴定;MTT法观察褪黑素(1×10-3～10-9mol/L)干预对细胞生长的影响;细胞分空白对照组、褪黑素10-8mol/L处理组和褪黑素10-6mol/L处理组,RT-PCR法观察细胞IL-7mRNA的表达;2.选用清洁级雄性SD大鼠110只,分为正常对照组、假手术对照组、松果体摘除组、松果体摘除+褪黑素7.5mg/(kg.d)腹腔注射组和松果体摘除+褪黑素15mg/(kg.d)腹腔注射组。术后4周和8周取材,运用免疫组织化学和RT-PCR方法观察胸腺上皮细胞IL-7表达的变化。结果褪黑素干预对大鼠胸腺上皮细胞的生长无显著影响,但能使IL-7mRNA的表达水平呈剂量依赖性升高;松果体摘除对大鼠胸腺上皮细胞表达IL-7无显著影响,补充褪黑素15mg/(kg.d)4周后IL-7表达显著增高,8周后均下降至正常水平。结论松果体及褪黑素可能通过影响大鼠胸腺上皮细胞IL-7的表达,从而调节胸腺细胞的分化发育。Objective To study the effect of pinealectomy and melatonin on interleukin 7 （IL-7） expression of rat thymic epithelial ceils （TECs） and its physiological significance. Methods Cultured rat TECs were immunostained with anti-pancytokeratin; MTT was applied to investigate the growth of cultured rat TECs with melatonin treatment （1 × 10^-3 - 10^-9 mol/L）, semiquantitative RT-PCR was applied to assess IL-7 mRNA expression. Totally 110 clean-grade male SD rats were divided into five groups： normal control group, sham operation group, pinealeetomized group, pinealeetomy ＋ 7.5mg/（kg·d） MLT group, pinealectomy ＋ 15mg/（kg·d） MLT group, and took the thymus out in the 4th week and the 8th week, lmmunohistoehemistry and semiquantitative RT-PCR were applied to observe IL-7 expression in rat TECs. Results Melatonin made no difference on rat TECs growth, but IL-7 mRNA expression in TECs was increased by melatonin treatment in a dose dependent manner; Pinealectomy made no difference on the synthesizing of IL-7 by rat TEC, but 15mg/（kg·d） melatonin treatment resulted in significant increasing of IL-7 expression in certain period. Conclusion pinealgland and melatonin may regulate the differentiation and development of thymocyte by IL-7 expression of TEC.

关 键 词：松果体 褪黑素 胸腺上皮细胞 白细胞介素7 免疫组织化学 反转录-聚合酶链反应 大鼠 

分 类 号：Q457[生物学—生理学]