肝细胞核因子6在小鼠肝内胆管发育过程中的表达  

EXPRESSION OF HEPATOCYTE NUCLEAR FACTOR 6 DURING THE PROCESS OF INTRAHEPATIC BILIARY DEVELOPMENT

作　　者：蒋吉英[1] 于树娜[1] 齐安东[1] 张杰[1] 王箐[1] 王晓萃[1] 张皓云[1] 李洪先[1] 

机构地区：[1]潍坊医学院人体解剖学教研室,山东潍坊261042

出　　处：《解剖学报》2008年第6期919-922,共4页Acta Anatomica Sinica

摘　　要：目的探讨肝细胞核因子6(HNF6)在肝内胆管发育过程中的作用。方法用RT-PCR及免疫组织化学等方法检测HNF6在小鼠胚胎发育的各个阶段以及成年肝中的表达。结果RT-PCR结果显示,HNF6mRNA的表达在E9d开始出现,与肝芽形成的时间吻合,E13dHNF6mRNA的表达消失,E15d又重新出现,并一直维持到出生。免疫组织化学显示,CK19免疫反应在E13d开始出现,此时免疫反应阳性细胞在肝索内散在分布。E15d时在近肝门处的门管区开始出现由单层的、CK19阳性细胞组成的胆管板,之后,阳性细胞反应主要分布于胆管板和小叶间胆管。E9～11d,多数肝索细胞呈HNF6阳性反应,E13d的肝索中未观察到HNF6的表达。E15～17d,HNF6阳性反应的分布与CK19相似,成年小鼠肝的胆管上皮细胞仍呈HNF6阳性反应。结论HNF6可能与肝干细胞的特化关系不大,而与肝发育的启动、肝干细胞向胆管上皮细胞的分化及其分化状态的维持有关。Objective To investigate the function of hepatocyte nuclear factor 6 （HNF 6 ） during the development of intrahepatic biliary. Methods The expression of HNF6 at different stages of intrabepatic bile ducts embryonic development and in the adult mouse liver were detected by RT-PCR and immunohistochemical staining. Results RT-PCR results showed that the expression of HNF6 was detected in the liver at E9d, the time of liver formation onset. Then, HNF6 disappeared transiently at E13d, but it appeared again at E15d. Its expression lasted till birth. According to the immunohistoehemical staining studies, the immunohistochemical positive reaction of CK19, which dispersed in the liver cords, appeared at E13d. At E15d, CK19-positive cells formed a single cell layer called the ductal plate around the branches of the portal vein located close to the hilum. Later, these immunohistoehemical positive cells were confined to the duetal plate and biliary epithelial cells. The immunohistoehemical positive reaction of HNF6 was detectable in most cells of liver cords at E9-11d, which became undetectable at E13d. Similar to CK19 at E15-17d, the immunohistochemical positive reaction of HNF6 could be observed at Pld. Conclusion HNF6 has no important role in the specification of liver during rat embryonic development . It may play a role in the onset of liver development, the differentiation of hepatic stem cell towards biliary epithelial cells and the maintenance of the morphological characteristic of biliary epithelial cells.

关 键 词：肝内胆管 胚胎发育 肝细胞核因子6 反转录-聚合酶链反应 免疫组织化学 小鼠 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]