升主动脉瘤明胶酶活性变化的实验研究  

EXPERIMENTAL STUDY ON THE CHANGES OF GELATINASE ACTIVITY OF ASCENDING AORTIC ANEURYSMS

作　　者：李想[1] 柏树令[2] 范军[2] 王军[2] 佟浩[2] 

机构地区：[1]中国医科大学细胞生物学教研室 [2]中国医科大学解剖学教研室,沈阳110001

出　　处：《解剖学报》2008年第6期941-943,共3页Acta Anatomica Sinica

基　　金：辽宁省自然科学基金资助项目(20051033)

摘　　要：目的探讨明胶酶在升主动脉瘤形成过程中的活性变化及意义。方法将35只Wistar大鼠随机分为对照组和实验组。采用升主动脉缩窄鼠制备升主动脉瘤模型。于术后3～5个月取升主动脉,应用明胶酶谱分析及薄膜原位酶谱法检测动脉瘤明胶酶的活性改变。结果正常动脉壁外膜无或极少有明胶酶活性;动脉瘤壁的中膜与外膜明胶酶活性明显增强。结论明胶酶活性升高可能在升主动脉瘤形成过程中起重要作用。Objective To investigate the activity changes of gelatinase in the formation of ascending aortic aneurysm. Methods Thirty five young Wistar rats were divided into two groups： the control group and the experiment group. The rat models induced by ascending aorta banding were made. The ascending aortas were taken after 3-5 months operation, changes of gelatinase activity was observed by gelatin zymography and film in situ zymography. Results Gelatinase activity of ascending aortic aneurysm was significantly increased compared with that of normal ascending aortic aorta. Conclusion Elevation of gelatinase activity may play a significant role in the formation of ascending aortic aneurysm.

关 键 词：升主动脉瘤 明胶酶谱分析 薄膜原位酶谱法 大鼠 

分 类 号：R654.3[医药卫生—外科学]