胃造瘘置管给药辅以饮食调控制备猪肝硬化模型  

Preparation of porcine model of hepatic cirrhosis by gastrostomy tube assisted with diet control

作　　者：张俊晶[1] 孟兴凯[1] 岳根全[1] 钟海燕[1] 张瑞芳[1] 

机构地区：[1]内蒙古医学院第一附属医院外科,呼和浩特010059

出　　处：《中华肝胆外科杂志》2008年第12期866-868,共3页Chinese Journal of Hepatobiliary Surgery

基　　金：基金项目：内蒙古医学院青年基金及内蒙古医学院重大课题基金（项目编号：NM2003ZD001）联合资助

摘　　要：目的探讨胃造瘘置管给药辅以饮食调控制备猪肝硬化模型的可行性。方法以普通猪作为研究对象，行暂时性胃造瘘术，四氯化碳按照0．25ml／kg，配以等量的脂肪乳，经T型管注入，30％食用乙醇60ml冲洗管道，一周2次；单纯玉米面饮食，5％乙醇水为惟一饲料。观察一般情况，定期切取小块肝组织作HE和VG染色并监测门静脉压，间断抽血化验肝功、肾功、白蛋白、胆红素等指标。结果10～12周后，肝硬化形成率88．9％，死亡率10％；ALT、AST在用药早期呈逐渐上升趋势，后期ALT、AST趋于平稳，并有下降的趋势，总胆红素持续上升；白蛋白持续下降；门静脉压早期呈下降趋势，后期迅速上升。结论（1）肝硬化模型成功率较高，造模时间短，死亡率较低。（2）病理活检仍是目前确诊肝硬化的金标准；ALT、AST变化对于评价肝硬化的进展无意义；白蛋白、总胆红素水平在肝硬化形成不同阶段具有统计学差异，对于评价肝硬化进程具有一定的价值。（3）胃造瘘置管给药辅以饮食调控制备猪肝硬化模型可行性较高，腹腔粘连较轻，适合于下一步的手术学研究。Objective To explore the feasibility of preparation of porcine model of hepatic cirrhosis by gastrostomy tube assisted with diet control. Methods The common pigs were chosen as experimental animals. Through the gastrostomy tube, CCl4 diluted with identical capacity of fat milk was administered at 0.25ml/kg. Then the tube was washed with 60 ml 30% edible alcohol twice a week. The pigs＇ diet was simple corn and 5% alcohol-water mixture. During the course of the experiment, little pieces of the hepatic tissue were extracted for HE staining and VG staining. The blood samples were collected to regularly test the function of liver and kidney, albumin, biliruhin. Results After 10-12 weeks, the rate of success was 88.9% and the mortality 10%. The levels of ALT and AST went up step by step in the early stage, then steadily increased. The level of TBIL increased continuously. However, the level of albumin decreased continuously. Conclusion 1） Rate of success of Model of hepatic cirrhosis is more higher and time is shorter. 2） Pathological biopsy is still a gold standard of diagnosing fibrosis at present. The changes of ALT and AST have no significance for evaluating the development of hepatic cirrhosis. Albumin and bilirubin have obviously difference before and after liver cirrhosis, which is useful to evaluate liver cirrhosis. 3）The feasibility of preparation of porcine model of hepatic cirrhosis by gastrostomy tube assisted with diet control is confirmed. We can hardly find any intestinal adhesion in these models, which is very meaningful to our further surgical surveys.

关 键 词：肝硬化 四氯化碳 动物模型 饮食控制 

分 类 号：R686[医药卫生—骨科学]