四川地区部分人群和与人接触密切的家畜禽戊型肝炎病毒感染的调查及基因分型的研究  

Antibody detection and sequencing analysis of Hepatitis E Virus in human population, swine and chicken in Sichuan region in China

作　　者：江永珍[1] 鲁健[1] 张丽萍[2] 田瑞光[1] 刘青恋[3] 毕胜利[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,北京100052 [2]四川科学城医院 [3]四川省疾病预防控制中心

出　　处：《中华实验和临床病毒学杂志》2008年第6期468-471,共4页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的了解四川地区人群和与人接触密切的家畜猪和鸡戊型肝炎（戊肝）病毒感染的分布及病毒基因分析。方法采用万泰生物药业的HEV EIA诊断试剂检测人群、猪和鸡血清中戊肝病毒（HEV）IgG抗体。采用逆转录套式PCR方法（RT-nPCR）检测猪胆汁和血清中戊肝病毒核酸，并对阳性产物进行纯化和部分核苷酸序列分析。结果学龄前儿童血清672份中有41份阳性，阳性率6．1％，成人血清661份中有280例阳性，阳性率42．36％，猪血清36份中有32份阳性，阳性率为88．89％，59份鸡血清HEV抗体均阴性。对15份HEV IgM抗体阳性的患者血清和54份猪胆汁进行RT-nPCR，从1份患者血清和3份猪胆汁扩增到戊肝病毒核酸，4株核苷酸序列之间同源性92．1％～98．6％，与GenBank HEV参考株ORF2相对应序列比较，核苷酸序列与Ch-T21（我国散发性急性肝炎人分离株HEV4B亚型）之间同源性最高占90．1％～96．9％。结论四川地区部分人群和与人接触密切的家畜猪均存在HEV感染，尤其是家畜猪感染率高达90％左右，从人和猪分离到的病毒株核苷酸同源性很高，均属于戊肝病毒基因4型。Objective To investigate the seroprevalence of HEV infection and genotype. Methods ELISA were used for detecting anti-HEV IgG of the serum samples, the nested reverse transcriptase PCR （RT- nPCR） was used for detecting HEV RNA in patient serum and swine bile samples. All samples were collected in 2005-2007 in some districts in Sichuan province. The primers used for genotyping were the ORF2 region of HEV genome. Results The anti-HEV IgG was detected positive in childrens 6.10% （41/672）, adults 42.26% （280/ 661 ）, swines 88.89% （32/36）, chickens negative（0/59）. 1 case of 15 serum samples of anti-HEV IgM positive and 3 of 54 swine bile samples were positive for HEV RNA by RT-PCR. Sequence analysis of 4 isolates has 92.1% to 98.6 % nucleotide sequence homology. These isolates from human and swine were identified closely related to Ch-T21 strain 90.1%-96.9% sequence homology, which belonged to HEV genotype 4B. Conclusions The swine were the risk factors in the spread of hepatitis E virus.

关 键 词：肝炎病毒 戊型 基因型 流行病学 

分 类 号：R51[医药卫生—内科学]