运用细胞培养／链特异性逆转录聚合酶链式反应法进行甲肝灭活疫苗的灭活验证试验  

Effective inactivatian test of inactivated hepatitis A vaccine using integrated cell culture/strand-specific reverse transcriptase-polymerase chain reaction

作　　者：周健[1] 姬光[1] 温嘉纳[1] 李佳[1] 沈伟[1] 郭自全[1] 廖国阳[1] 姜述德[1] 孙明波[1] 

机构地区：[1]中国医学科学院北京协和医学院医学生物学研究所,昆明650118

出　　处：《中华实验和临床病毒学杂志》2008年第6期488-491,共4页Chinese Journal of Experimental and Clinical Virology

基　　金：云南省自然科学基金资助（基金编号20040030Q）

摘　　要：目的建立一种快速灵敏特异的甲肝灭活疫苗的灭活验证方法。方法运用细胞培养／链特异性逆转录聚合酶链式反应对甲肝灭活疫苗进行灭活验证，并与传统的ELISA法及巢式RT-PCR检测甲肝全RNA进行比较。结果细胞培养／链特异性逆转录聚合酶链式反应法对甲肝灭活疫苗进行灭活验证，结果全为阴性，与传统的ELISA法结果相同。而采用巢式RT-PCR检测甲肝全RNA的方法对甲肝灭活疫苗进行灭活验证，则出现一些假阳性结果。结论细胞培养，链特异性逆转录聚合酶链式反应法是一种简便、快速、灵敏的甲肝灭活疫苗的灭活验证方法。大大缩短了检测周期，用于疫苗的常规检测有较好前景。Objective To establish an quick, sensitive and specific assay for effective inactivatian test of inactivated hepatitis A vaccine. Methods effective inactivatian test of inactivated hepatitis A vaccine were carried out using integrated cell culture/strand-specific RT-PCR （ICC/strand-specific RT-PCR） assay compared with traditional ELISA and nest RT-PCR assay.Results all the samples were infectious negative detecting by both ICC/ strand-specific RT-PCR and ELISA assay, while some samples appeared false positive detecting by nest RT-PCR. Conclusion ICC/strand-specific RT-PCR assay is a novel, rapid, sensitive and reliable method for effective inactivafian test of inactivated hepatitis A vaccine. Shorting detection period largely, this assay may be used as an alternative method for routine inactivated hepatitis A vaccines test.

关 键 词：肝炎 甲型 疫苗 灭活 细胞培养 逆转录聚合酶链反应 

分 类 号：R186[医药卫生—流行病学]