老幼龄扬子鳄端粒相对长度的差异性分析  

Analyzing the differences in the relative lengths of DNA telomeres between the aging and the neonatal Chinese alligators

作　　者：徐敏[1] 吴孝兵[1] 

机构地区：[1]安徽师范大学生命科学学院,重要生物资源保护与利用安徽省重点实验室,芜湖241000

出　　处：《动物学报》2008年第6期1119-1123,共5页ACTA ZOOLOGICA SINICA

基　　金：国家自然科学基金(30770312);"动物生物学"安徽师范大学优秀团队专项基金;安徽省学术与技术带头人专项基金及生物环境与生态安全安徽省高校省级重点实验室基金资助~~

摘　　要：To assess the differences in the relative lengths of the DNA telomere between the matured and the adolescent Chinese alligators Alligator sinensis and to offer the possible molecular evidence for judging the age of the Chinese alligators,we applied the PCR method to amplify the telomere DNA of both the matured and the juvenile Chinese alligators in our research.Subsequently,we analyzed and compared the outcomes of the two groups by using a Semi-Quantitative Technique.The results of our analyses show that the relative average gray value of the blood cells’ telomere PCR products of the adolescent group is higher than that of the matured group.These analytical results are indications that the blood cells’ telomere DNA sequence of the adolescent Chinese alligators is longer than that of the matured Chinese alligators.Our paper will also discuss the feasibility of the use of the PCR method to determine the relative lengths of the telomere.To assess the differences in the relative lengths of the DNA telomere between the matured and the adolescent Chinese alligators Alligator sinensis and to offer the possible molecular evidence for judging the age of the Chinese alligators, we applied the PCR method to amplify the telomere DNA of both the matured and the juvenile Chinese alligators in our research. Subsequently, we analyzed and compared the outcomes of the two groups by using a Semi-Quantitative Technique. The results of our analyses show that the relative average gray value of the blood cells＇ telomere PCR products of the adolescent group is higher than that of the matured group. These analytical results are indications that the blood cells＇ telomere DNA sequence of the adolescent Chinese alligators is longer than that of the matured Chinese alligators. Our paper will also discuss the feasibility of the use of the PCR method to determine the relative lengths of the telomere [Acta Zoologica Sinica 54 （6）： 1119- 1123, 2008].

关 键 词：扬子鳄 端粒 年龄 聚合酶链反应 

分 类 号：Q953[生物学—动物学]