早期高压氧、低气压干预对低气压环境大鼠降钙素基因相关蛋白和内皮素的影响  

Effects of early hyperbaric oxygen and hypobaric intervention on the levels of calcitonin gene related protein and endothelin in rats in hypobaric environment

作　　者：潘树义[1] 潘晓雯[1] 杨晨[1] 李铭鑫[1] 张禹[1] 孟祥恩[1] 刘薇[1] 钟文[2] 李保国[3] 

机构地区：[1]海军总医院,北京100037 [2]解放军第四二一医院 [3]北京世纪坛医院

出　　处：《中华航海医学与高气压医学杂志》2008年第6期325-328,共4页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

摘　　要：目的探讨早期高压氧、低气压干预对低气压环境大鼠降钙素基因相关蛋白（calcitonin gene related protein，CGRP）和内皮素（endothelin，ET）的影响，对比研究两种方法的作用。方法62只Wistar大鼠随机分成6组：（1）对照组，（2）海拔3800m（2h）组，（3）海拔3800m（3d）组，（4）海拔3800m（7d）组，（5）高压氧干预组，（6）低气压环境干预组。首先把（2）、（3）、（4）实验组大鼠置于低压氧舱中，匀速减压使舱内压力由海平面0．1MPa降至0．062MPa，相当于海拔3800m的高原低气压环境，在2h、3d、7d时间点断头取血备检；对（5）组大鼠先期给予高压氧处理（0．2MPa，1次／d，吸氧60min，连续5d），第6天，再置于低压氧舱中匀速减压至0．062MPa，2h后断头取血备检；对（6）组大鼠先期给予低气压缺氧处理（0．062MPa，1次／d，2h／次，连续5d），第6天，再置于低压氧舱中匀速减压至0．062MPa，2h后断头取血备检；将上述不同处理方法所得血样离心取上清，放免法测定CGRP、ET浓度。结果与对照组比较，CGRP浓度随着大鼠在低气压环境中的时间延长有逐渐减少趋势，但差异无统计学意义，高压氧预处理对CGRP无影响，低气压缺氧预处理虽然使CGRP浓度下降趋势更明显，但差异无统计学意义。ET浓度随着时间的延长有逐渐增加趋势，但也无统计学意义。高压氧[（305．26±39．66）μg/L]、低气压缺氧预处理[（309．18±25．07）μg/L]均使ET浓度明显升高，差异有统计学意义（P〈0．05）。结论高压氧、低气压缺氧早期干预主要影响大鼠ET的变化，高压氧预处理可能具有增强机体高原适廊能力的作用。Objective To investigate effects of early hyperbaric oxygen and hypobaric intervention on the levels of caleitonin gene related protein （CGRP） and endothelin （ET） in rats in hypobaric environment, and to compare the effects of two methods. Methods The experimental Wistar rats were randomly divided into six groups： （1） Control group, （2） 3800 m （2 h） group, （3） 3800 m （3 d） group, （4） 3800 m （7 d） group, （5） Hyperbaric oxygen intervention group, （6） Hypobarie environment intervention group. Firstly, the rats in the second, third and fourth groups were plaeed in a hypobarie oxygen chamber, in which the pressure was decreased at equal speed from 0. 1 MPa to 0. 062 MPa, which corresponded the pressure at the 3800 m altitude. After two hours and the 3rd, 7th days, the rats were decapitated to obtain blood for later examination. Secondly, the rats in the fifth group received hyperbaric oxygen treatment （0.2 MPa, once per day, oxygen inhalation for 60 minutes, successively 5 day, s）. On the sixth day, the rats were placed in a hypobarie oxygen chamber, in which the pressure was decreased at equal speed to 0. 062 MPa. After two hours, the rats were decapitated to obtain blood for later examination. Thirdly, the rats in the sixth group received hypobafic hypoxia treatment （0.062 MPa, once per day, 2 h once, for successively 5 days）. On the sixth day, the rats were placed in a hypobaric oxygen chamber, in which the pressure was decreased from equal speed to 0. 062 MPa. After two hours, the rats were decapitated to obtain blood for later examination. The blood samples obtained fi＇om above different treatment methods were centrifuged to obtain the supernatant, and the levels of CGRP and ET were measured by radioimmunology method. Results Compared with control group, CGRP level decreased in tendency with time extension, but there was no significant difference between of them. The hyperbaric oxygen treatment had no effect on CGRP level, but hypobaric hypoxia pretreatment

关 键 词：高压氧 高原病 低气压 降钙素基因相关蛋白 内皮素 

分 类 号：R5[医药卫生—内科学]