聚合酶链反应在诊断肺结核中的应用  

Polymerase Chain Reaction for the Diagnosis of Pulmonary Tuberculousis

作　　者：刘双[1] 姚天樵[1] 徐利[1] 祁雅慧[2] 殷红[2] 姚庆英[2] 赫翠珍[2] 高天祥[2] 

机构地区：[1]首都医科大学附属北京安贞医院呼吸内科 [2]首都医科大学实验中心

出　　处：《首都医科大学学报》1998年第1期31-33,共3页Journal of Capital Medical University

摘　　要：应用聚合酶链反应（ＰＣＲ）技术对７８例患者的痰、支气管肺泡灌洗液（ＢＡＬＦ）和胸水标本进行结核菌检测；并同时进行涂片抗酸染色、皮肤结核菌素试验及血结核菌抗体检测。结果：ＰＣＲ阳性率为４６．１５％，与涂片镜检、结核菌素试验强阳性、血结核菌抗体阳性率（分别为３．８５％、１７．９５％、１１．５４％）相比均有显著性差异（Ｐ＜０．０５）。由此可见ＰＣＲ方法比传统方法具有更高的结核杆菌检出率。olymerase chain reaction(PCR) was applied to detect DNA of mycobacterium tuberculosis(TB) in 78 clinical samples. Methods: For each sample, TBPCR, the positive rate of acidfast staining, tuberculin skin test and tuberculous antibody were made. The results of PCR were compared with those of conventional procedures and clinical diagnosis standard. Results: It was found that the positive rate of acidfast staining, tuberculin skin test, tuberculous antibody and PCR were 3.85%、17.95%,11.54% and 46.15% respectively in 78 tuberculous samples. The sensitivity of PCR was much higher than the former diagnostic procedures. Conclusions: up to now, the results showed that the PCR was a valuable detective tool for diagnosis and differential diagnosis of tuberculosis. Diagnosis of tuberculousis still need to be determined by the patient's chlinical menfestions and others methods.

关 键 词：聚合酶链反应 结核杆菌 肺结核 

分 类 号：R521.04[医药卫生—内科学]