酶活性测定法检测致病性大肠杆菌对小鼠肠上皮细胞的粘附  

Detection of EPEC Adherence to Endotheliocyte from Murine Gut by ONPG Method

作　　者：崔光辉[1,2] 张颖[1,2] 管远志[1,2] 

机构地区：[1]中国协和医科大学基础医学院 [2]中国医学科学院基础医学研究所

出　　处：《基础医学与临床》1998年第1期20-22,19,共4页Basic and Clinical Medicine

基　　金：国家自然科学基金

摘　　要：本文介绍了一种以小鼠肠上皮细胞为靶细胞，以大肠杆菌的半乳糖苷酶活性来反映粘附菌量的体外模型。此模型能准确地表现致病性大肠杆菌（ＥＰＥＣ）对宿主肠道上皮细胞的粘附状况，并具有操作简便，客观性强的特点，适用于对ＥＰＥＣ粘附及抗粘附机制的研究。A new cell model made of endotheliocyte from murine gut was set up for the detection of adherence of enteropathogenic E.Coli (EPEC). The binding and adherence of the bacteria to the target cells was examined quantitativly by ONPG methol. The cell model in our experiment showed better results as the real mammalian target cells comparing that with Hep2 cell line in the research of EPEC pathogenesis.

关 键 词：大肠杆菌 粘附 半乳糖苷酶 肠上皮细胞 酶活性 

分 类 号：R378.2[医药卫生—病原生物学]