全反式维A酸对血管平滑肌细胞增殖,细胞内钙离子和氢离子含量的影响  

Effect of all trans retinoic acid on proliferation, intracellular free calcium content and intracellular pH of rabbit vascular smooth muscle cells1

作　　者：余晨光[1] 章鲁[1] 顾培坤[1] 魏丕敬[1] 金正均[1] 

机构地区：[1]上海第二医科大学神经生物学教研室

出　　处：《中国药理学与毒理学杂志》1998年第1期73-74,共2页Chinese Journal of Pharmacology and Toxicology

基　　金：上海市高等学校科学技术发展基金

摘　　要：全反式维Ａ酸对血管平滑肌细胞增殖，细胞内钙离子和氢离子含量的影响１余晨光章鲁２顾培坤３魏丕敬３金正均３（上海第二医科大学神经生物学教研室，２计算机室，３药理学教研室，上海２０００２５）血管平滑肌细胞（ｖａｓｃｕｌａｒｓｍｏｏｔｈｍｕｓｃｌｅｃｅｌｓ，...The effect of all trans retinoic acid (ATRA) on changes of DNA synthesis, intracellular free calcium content ([Ca2+]i) and intracellular pH (pHi) of cultured rabbit vascular smooth muscle cells (VSMC) induced by fetal calf serum (FCS) were studied by thymidine incorporation, Fura2/AM and BCECF/AM fluorescent probe mark and digital image processing techniques. The results showed that (1) ATRA (0.01, 0.1 and 1.0 μmol·L1) significantly inhibited 20% FCSinduced VSMC DNA synthesis and its maximal inhibitory rate at 1 μmol·L1 was 90%; (2) incubated with 20% FCS for 5 min, VSMC [Ca2+]i increased and VSMC i decreased. These effects of FCS were significantly inhibited by ATRA 0.1 μmol·L1 preincubated for 10 min. The results indicate that ATRA lowering VSMC [Ca2+]i and pHi may be attribute to inhibition of the proliferation of VSMC.

关 键 词：全反式维A酸 血管平滑肌 细胞增殖 钙 氢 

分 类 号：R977.21[医药卫生—药品] R972[医药卫生—药学]