神经肽Y对培养的自发性高血压大鼠动脉平滑肌细胞的影响  

Effect of Neuropeptide Y on the Cultured Arterial Smooth Muscle Cells in Spontaneously Hypertensive Rats

作　　者：吴翠环[1] 任大宏[1] 陈多恩[1] 

机构地区：[1]同济医科大学基础医学院病理学教研室,武汉430030

出　　处：《同济医科大学学报》1998年第1期19-21,共3页Acta Universitatis Medicinae Tongji

基　　金：国家自然科学基金资助项目(No.39370287)

摘　　要：应用细胞培养，＾３Ｈ－胸腺嘧啶核苷和＾３Ｈ－亮氨酸的掺入及细胞计数方法，研究缩血管物质神经肽Ｙ对血管平滑肌细胞生长的作用。结果发现，用神经肽Ｙ作用４８ｈ后可促进离体培养的自发性高血压大鼠动脉平滑肌细胞ＤＮＡ合成增加２８．０％，蛋白质合成增加５４．６％，与对照组比较有显著性差异；而细胞数无明显增加。We applied the method of cellular culture, tritiated thymidine and leucine incorporation, and calculation in cell number to investigate the role of vasoconstrictor substance (neuropeptide Y NPY) in vascu-lar smooth muscle cell (VSMC) growth. It was observed that 48-hour exposure to neuropeptide Y for these cells caused an 28. 0 % and 54. 6 % increase respectively in DNA and protein synthesis in cultured VSMC in SHR (compared with serum control P<0, 01), but no significant increase in cell number (P>0. 05). The re-sults demonstrate that NPY accelerates VSMC hypertrophy in SHR, and an increase in density of NPY-con-taining innervation in the vasculature of SHR is one of the important factors in the structural changes of hy-pertensive vessels.

关 键 词：神经肽Y 自发性 高血压 肥大 平滑肌细胞 

分 类 号：R544.1[医药卫生—心血管疾病] Q516[医药卫生—内科学]