不同频率电针耐受对κ阿片受体mRNA转录的影响  

EFFECTS OF 2 Hz and 100 Hz ELECTROACUPUNCTURE TOLERANCEON THE TRANSCRIPTION OF CNS κ OPIOID RECEPTOR mRNA

作　　者：王韵[1] 王晓民[1] 韩济生[1] 

机构地区：[1]北京医科大学神经科学研究中心

出　　处：《北京医科大学学报》1998年第1期1-4,共4页Journal of Peking University(Health Sciences)

基　　金：国家自然科学基金

摘　　要：目的：研究２Ｈｚ及１００Ｈｚ电针耐受对κ受体ｍＲＮＡ转录的影响。方法：每天给大鼠２Ｈｚ及１００Ｈｚ电针１次，每次３０ｍｉｎ，连续电针６ｄ，在电针的第１、３、６天分别取各个脑区及脊髓，提取ＲＮＡ，然后用反转录聚合酶链反应（ＲＴＰＣＲ）方法检测κ受体ｍＲＮＡ水平。结果：（１）电针后的第１天，各脑区及脊髓κ受体ｍＲＮＡ均无明显改变；（２）每天给大鼠针刺（不给电刺激）１次，连续多次针刺可使纹状体内κ受体ｍＲＮＡ显著增加，在针刺的第６天杏仁核内κ受体ｍＲＮＡ也轻度增加；（３）每天给大鼠１００Ｈｚ电针１次，每次３０ｍｉｎ，连续电针３～６次可使脊髓中κ受体ｍＲＮＡ水平下降，同样现象也可在纹状体及杏仁核中观察到，２Ｈｚ电针的结果很不一致，它使ＰＡＧ和纹状体内κ受体ｍＲＮＡ水平降低，而杏仁核和脊髓中的κ受体水平增强，或呈双相反应。结论：单纯针刺３～６ｄ本身有一定的中枢效应，但是多次电针则在大多数情况下抑制κ受体ｍＲＮＡ的转录，提示内源性阿片肽大量释放也可引起阿片受体下调，这可能是电针耐受的机制之一。Objective: To observe the effects of 2 Hz and 100 Hz electroacupuncture(EA) stimulation on the transcription of CNS κ opioid receptor mRNA. Methods: EA in 2 Hz or 100 Hz was administered 30　min once a day for 6 days, total RNA extracted from discrete brain regions and the spinal cord on the first, third, sixth days and the level of mRNA detected with the method of reversetranscriptionpolymerase chain reaction(RTPCR). Results: (1) No significant change of the abundance of κ receptor mRNA was observed in various brain areas and spinal cord 24　h after one session of EA stimulation. (2)In rats receiving acupuncture without electricstimulation once (30 min) every day for 6 days, the mRNA level of κ opioid receptor increased significantly in the striatum, as well as in amygdala on the 6th day. (3)A significant decrease of the κ　receptor mRNA level was found after 3～6 sessions of 100 Hz EA stimulation in the spinal cord, striatum and amygdala. In contrast, changes induced by repeated 2 Hz EA stimulation were not consistent, κ receptor mRNA level decreased in PAG and striatum, increased or changed biphasically in the amygdala and spinal cord. Conclusion: (1) Insertion of the acupuncture needle in the acupiont without giving eletric stimulation might produce certain CNS effects. (2)In most cases, repeated EA stimulations inhibited the transcription of κ opioid receptor t hat may lead to the downregulation of κopioid receptor. This may serve as one of the molecular mechanisms for the development of EA tolerance.

关 键 词：电针 针灸耐受 受体 阿片样 阿片受体 MRNA 

分 类 号：R245－0[医药卫生—针灸推拿学] R338.8[医药卫生—中医临床基础]