大鼠脑脊液、脊髓灌流液和脊髓背部单胺类递质及其代谢产物的含量比较  

THE CONTENTS OF MONOAMINE TRANSMITTERS AND THEIR METABOLITES IN RATCEREBROSPINAL FLUID, SPINAL PERFUSATE AND DORSAL HALF OF THE SPINAL CORD

作　　者：田津斌[1] 方明[1] 刘雪松 许伟[1] 韩济生[1] 

机构地区：[1]北京医科大学神经科学研究中心

出　　处：《北京医科大学学报》1998年第1期5-8,共4页Journal of Peking University(Health Sciences)

基　　金：国家自然科学基金

摘　　要：目的：比较大鼠脊髓单胺类神经递质的含量和基础释放量。方法：以反相离子高效液相色谱电化学检测法对样品中的单胺类递质及其代谢产物进行定量分析。结果：（１）分别测得了大鼠脑脊液、脊髓灌流液和脊髓背部组织提取液中单胺类递质及其代谢产物的含量；（２）在大鼠脊髓背部组织中同时含有５羟色胺、去甲肾上腺素和多巴胺，但在脊髓灌流液和脑脊液中只能检测到去甲肾上腺素，部分样品中能测到多巴胺；（３）递质代谢产物在脑脊液和脊髓灌流液中的含量均高于其对应的递质。结论：该方法的分辨率和灵敏度均能满足实验要求，所得数据为进一步研究脊髓水平单胺类递质的代谢和生理功能奠定了基础。Objective: To compare the contents and the basic releases of monoamine transmitters in rat spinal cord. Methods: By using reversedphase high performance liquid chromatography with electrochemical detector to separate and measure monoamine transmitters and their metabolites. Results: (1) Obtained the levels of monoamine transmitters and their metabolites in the cerebrospinal fluid (CSF), spinal perfusate and dorsal half of the spinal cord; (2) Observed that 5HT, NA and DA existed in the dorsal half of the spinal cord, but only NA could be detected in all samples of the CSF and spinal perfusate, DA could be detected in part of samples; (3) The contents of the metabolites of monoamine transmitters are higher than their corresponding transmitters in the CSF and spinal perfusate. Conclusion: The sensitivity and resolution of the method are satisfactory for the aim of the present study and the data from this experiment provide a basis for the further study of the metabolism and physiological functions of monoamine transmitters in the spinal cord.

关 键 词：脑脊液 脊髓 灌流液 去甲肾上腺素 多巴胺 

分 类 号：Q592.5[生物学—生物化学] Q577