聚合酶链反应检测HGV RNA方法的建立及应用  被引量:7

DEVELOPMENT AND APPLICATION OF POLYMERASE CHAINREACTION METHOD FOR DETECTION OF GB VIRUS C/HEPATITIS G VIRUS

在线阅读下载全文

作  者:常锦红[1] 王豪[1] 杜绍财[1] 孙焱[1] 陶其敏[1] 

机构地区:[1]北京医科大学人民医院肝病研究所

出  处:《北京医科大学学报》1998年第1期12-14,共3页Journal of Peking University(Health Sciences)

摘  要:目的:了解我国不同人群中庚型肝炎病毒(GBVC/HGV)感染的状况。方法:以NS5区序列分析为基础设计引物,建立聚合酶链反应方法,并对我国几个地区、不同疾病状况的268例患者进行庚型肝炎病毒核酸的检测。结果:有输血史的健康人群、HBV和HCV感染者及慢性非乙非丙型肝炎患者的GBVC/HGVRNA阳性率均高于自然人群,分别为13.3%,18.4%,19.8%,8.9%,其中有明确血液暴露史者的检出率达20.1%。结论:庚型肝炎在我国有广泛的流行,输血等肠道外途径为重要危险因素。Objective: To investigate the infection of GB virus C/hepatitis G virus (GBVC/HGV) in Chinese population. Methods: We established a detection system using reverse transcriptase polymerase chain reaction (RTPCR) on the basis of sequence analysis of the nonstructural gene 5 (NS5) region of GBVC/HGV genome, which was applied in detection of viral RNA in 268 patients from different geographic areas of our country and with different liver diseases. Results: The positive rates of GBVC/HGV RNA in healthy people with history of transfusion, patients with HBV or HCV infection and chronic nonB, nonC hepatitis were higher than that of natural population (13.3%, 18.4%, 19.8% and 8.9% respectively). Among these patients, the group with history of blood exposure had high prevalence of infection (20.1%). Conclusion: GBVC/HGV infection is widespread in China, and transfusion is the main risk factor.

关 键 词:庚型肝炎病毒 RNA 聚合酶链反应 

分 类 号:R512.630.4[医药卫生—内科学] R446.9[医药卫生—临床医学]

 

参考文献:

正在载入数据...

 

二级参考文献:

正在载入数据...

 

耦合文献:

正在载入数据...

 

引证文献:

正在载入数据...

 

二级引证文献:

正在载入数据...

 

同被引文献:

正在载入数据...

 

相关期刊文献:

正在载入数据...

相关的主题
相关的作者对象
相关的机构对象