地塞米松引起小鼠胸腺内细胞凋亡的半薄切片观察  

OBSERVATION OF THE SEMITHIN SECTIONS OF THE MOUSE THYMICCELL APOPTOSIS INDUCED BY DEXAMETHASONE

作　　者：陈英杰[1] 孙品伟[1] 董静霞 毕振武 朱小辉 

机构地区：[1]北京医科大学组织学与胚胎学系

出　　处：《北京医科大学学报》1998年第1期18-20,共3页Journal of Peking University(Health Sciences)

基　　金：国家自然科学基金

摘　　要：目的：获取地塞米松引起小鼠胸腺细胞凋亡的时间变化曲线。方法：雄性ＢＡＬＢ／ｃ小鼠腹腔内注射地塞米松，油镜下观察甲苯胺蓝染色的半薄切片，计数１００ｍｍ２内细胞总数及凋亡细胞（Ａｃ）数和凋亡小体（Ａｂ）数及Ａｃ、Ａｂ百分率。结果：胸腺皮质与髓质形成Ａｃ、Ａｂ的高峰时相为１５ｈ。在皮质，１～１５ｈ为凋亡上升期，１５～１９ｈ为凋亡高峰平台期，１９～３０ｈ为凋亡下降期；在髓质，１～１５ｈ为凋亡上升期，１５ｈ为凋亡高峰期，１５～３０ｈ为凋亡下降期。结论：这一时间变化曲线为深入探讨胸腺的细胞凋亡提供数值变化资料。Objective:To obtain the time fluctuating curves of the mouse thymic cell apoptosis induced by dexamethasone. Methods: When male BALB/c mice were received an i.p. injection of dexamethasone (DEX), the mouse thymus apoptosis was induced. The thymuses were cut into the semithin sections, which were stained with the toluidine blue.The sections were examined under the oil object lens. The area of 100 mm2　was　selected to count　with the ocular microsurveyingmeter:apoptotic cell number (Ac),　apoptotic body number (Ab) and total cell number (T).According to the formula:Ac %=Ac/T, Ab%=Ab/T, the Ac percentage and Ab percentage at different time from the experimental　and the control groups were obtained. Results: Both of the peak time of apoptosis occurring in the thymic cortex and medulla were 15 h. In the cortex,the period of apoptosis ascending was 1～15 h, the period of apoptosis peak platform was 15～19 h and the period of apoptosis descending was 19～30 h. In the medulla, the period of apoptosis ascending was 1～15 h, the peak time of apoptosis was 15 h and the period of apoptosis descending was 15～30 h. Conclusion: The time fluctuating curves of Ac percentage and Ab percentage of the mouse thymic cortex and medulla offered the data for us to further study the variation of morphology of the thymic cell apoptosis and its mechanism.

关 键 词：地塞米松 药理学 胸腺 细胞凋亡 半薄切片 

分 类 号：R977.1[医药卫生—药品] R361.2[医药卫生—药学]