实验性脑损伤后肾脏自由基损害及其抗氧化酶的保护作用  

Changes of oxygen free radicals and protective effects of the antioxidases on the after brain injury

作　　者：郭乃芸[1,2,3,4] 孙长凯[1,2,3,4] 李元勋 罗晓阳[1,2,3,4] 

机构地区：[1]第四军医大学 [2]西京医院肾内科 [3]全军神经科学研究所 [4]解放军第四七三医院

出　　处：《第四军医大学学报》1998年第1期64-65,共2页Journal of the Fourth Military Medical University

摘　　要：目的：观察脑损伤后继发性肾脏自由基损害过程及其抗氧化酶的保护作用．方法：５０只成年雌雄各半Ｗｉｓｔａｒ大鼠，自由落体致伤造成重型颅脑损伤模型．用硫代巴比妥酸比色法，谷胱甘肽过氧化物酶（ＧＳＨｐｘ）活力测定法及铜锌超氧化物歧化酶（Ｃｕ／ＺｎＳＯＤ）放射免疫分析法检测正常对照组、伤后０５，６，２４，７２ｈ肾脏匀浆组织中脂质过氧化产物丙二醛（ＭＤＡ）和抗氧化酶ＧＳＨｐｘ，Ｃｕ／ＺｎＳＯＤ变化．结果：脑损伤后早期（０５ｈ）ＭＤＡ含量即增高（Ｐ＜００５），６，２４ｈ则显著增高（Ｐ＜００１）；ＧＳＨｐｘ和Ｃｕ／ＺｎＳＯＤ则均有所减少，以２４ｈ最为明显（Ｐ＜００１）．结论：脑损伤后继发性肾内自由基产生增多，抗氧化酶保护作用降低，可能是引起继发性肾损害的一个重要因素，也是临床早期使用抗氧化酶的理论依据之一．Aim:To study the effects of oxygen free radicals and antioxidase on secondary kidney damage due to brain injury Methods:Fifty adult Wistar rats were divided into 5 groups at random and model of brain injury was obtained by a free falling body Colorimetric examination and radioimmunoassay were used to measure malonadehyde(MDA),Cu/Zn superoxide dismutase(Cu/Zn SOD) and activity of glutathione peroxidase(GSHpx) of kidney homogenate of the subjects and the controls at 05,6,24 and 72 h after brain injury Results:MDA levels of subjects got higher than those of the controls[P<005(05 h),P<001(6,24 h)] There was significant decrease in GSHpx activity of the subjects at 24 h after the injury(P<001),Cu/Zn SOD level of the subjects reduced significantly 24 h after the injury(P<001) Conclusion:The balance between the generation of oxygen free radicals and decrease of the protective effects of the antioxidases may take part in the development of secondary kidney damage and early treatment with antioxidases may be benifical

关 键 词：脑损伤 肾损害 丙二醛 GSH-PX 超氧化物歧化酶 

分 类 号：R651.15[医药卫生—外科学] R692[医药卫生—临床医学]