中国人丙型肝炎病毒2a型基因组C区基因cDNA的克隆及序列分析  

Cloning and sequencing of core gene cDNA of hepatitis C virus derived from a patient with HCV infection in China

作　　者：赵小宁[1] 徐德忠 惠宏襄[1] 张景霞 

机构地区：[1]第四军医大学

出　　处：《第四军医大学学报》1998年第1期66-69,共4页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金

摘　　要：目的：测定陕西地区丙型肝炎病毒的Ｃ区基因序列，为进一步研究ＨＣＶ的流行病学和开展ＨＣＶ的诊治打下基础．方法：自行设计合成了两条正向引物和两条反向引物，用反转录ＰＣＲ（ＲＴＰＣＲ）法从陕西地区１例ＮＡＮＢ型肝炎患者血清中扩增出４３６ｂｐ的片段，将此片段克隆于ＰＧＥＭ７Ｚｆ（＋）中用全自动序列分析仪测序．结果：测得４３６ｂｐ的核苷酸序列，将所得核苷酸序列及由它们所编码的相应氨基酸序列与ＨＣＶＪ６，ＨＣＶＪ８，ＨＣＶＨｅｂｅｉ，ＨＣＶＨｕｎａｎ，ＨＣＶ１比较，显示所分离ＨＣＶ在核苷酸水平上与以上分离株的同源性分别为９５７％，８７２％，８３９％，８３９％和８２７％；在氨基酸水平上的同源性分别为９７７％，９４７％，９０８％，９０１％和９０１％．Aim: To clone and sequence core gene cDNA of hepatitis C virus in Shaanxi ChinaMethods: Two sense primers and antisense primers were designed and synthesized A 436 bp fragment in core region was amplified by reverse transcription polymerase chain reaction(RTPCR) The fragment was cloned into PGEM7Zf(+) , then sequenced by autosequencing instrument Results: The nucleotide sequence and corresponding deduced amino acid sequences were compared with those of HCVJ6, HCVJ8 , HCVHebei, HCVHunan and HCV1 The nucleotide sequence homology to those isolated strains were found to be 957%, 872%839%, 839% and 827% respectively;the amino acid sequence homology to those isolated strains were found to be 977%, 947%, 908%, 901% and 901% respectively Conclusion: The results indicates that the isolated strain is close to HCVJ6 and belong to type 2a

关 键 词：丙型肝炎病毒 病毒基因 克隆 序列分析 CDNA 

分 类 号：R512.63[医药卫生—内科学]