小鼠肠型放射病时小肠壁肥大细胞的动态变化及其与一氧化氮的关系  

The dynamic distribution of intestinal mucosal mast cells and their relationship with nitric oxide in the mice with intestinal type radiation sickness

作　　者：魏丽春[1] 马海昕[1] 范风云[1] 王志祥[1] 

机构地区：[1]第四军医大学西京医院放射治疗中心

出　　处：《第四军医大学学报》1998年第1期80-83,共4页Journal of the Fourth Military Medical University

摘　　要：目的：探讨肥大细胞在肠型放射病中的作用．方法：应用免疫组化、组织化学和常规组织染色方法，观察受辐照小鼠小肠壁肥大细胞、一氧化氮合酶（ＮＯＳ）阳性成分、组织结构及其变化．结果：受照后随着存活时间从２４ｈ，４８ｈ至７２ｈ的延长，小鼠出现进行性加重的肠管扩张、肠壁充血水肿和组织损伤坏死，肥大细胞出现脱颗粒、含着染颗粒的肥大细胞数目呈进行性下降，并伴随着肠壁ＮＯＳ活性或数量的增加．给予特异性ＮＯＳ抑制剂后，肥大细胞脱颗粒受到抑制，肠壁的病理改变明显减轻．结论：肠壁肥大细胞通过释放其胞浆颗粒活性物质参与肠型放射病病理过程；一氧化氮可能调节肥大细胞颗粒的释放；肥大细胞释放的活性物质如单胺和神经肽（如ＶＩＰ），可能是肠道放射病病理过程如肠壁充血。Aim:To investigate the significance of mucosal mast cells in the intestinal type radiation sickness and their relationship with nitric oxide Methods: Mucosal mast cells, nitric oxide synthase (NOS), tissue structure and their dynamic changes of intestines were examined by using immunohistochemical, histochemical and histological staining methods Results: After radiation the dilation of intestinal cavity, hyperaemia, edema and degeneration or injury of intestinal tissue were observed increasingly from 24 h to 72 h survival The number of mucosal mast cells, which contain stained granules, came decreasingly down, and degranulation of mast cells was noted In the mean time, NOS activity or NOS positive structures came increasingly up However, preadministration of NOS inhibitor (LNAME) held back the decrease of stained mucosal mast cell number and degranulation ofmast cells In this case, the intestinal pathological change alleviated obviousely Conclusion: Mucosal mast cells are involved in the pathlogical process of intestinal type radiation sickness of mice via releasing their cytoplasmic granular matter such as monoamines and peptides; and the degranulation of mast cells might be regulated by nitric oxide

关 键 词：肥大细胞 一氧化氮合酶 肠型放射病 放射病 病理 

分 类 号：R818.02[医药卫生—放射医学]