恒河猴异体椎间盘移植生物学活性和生物化学研究  

Study of histological activity and biochemistry of intervertibral disc allografting of rhesus monkey

作　　者：周勇[1,2] 阮狄克[1,2] 陆瓞骥[1,2] 沈根标[1,2] 孙宏慧[1,2] 

机构地区：[1]第四军医大学唐都医院骨科 [2]香港大学骨科学系

出　　处：《第四军医大学学报》1998年第1期84-86,共3页Journal of the Fourth Military Medical University

摘　　要：目的：通过异体恒河猴椎间盘移植生物学活性及生物化学观察，研究异体椎间盘移植能否存活及其可能归宿，为临床椎间盘疾病的治疗探索可能的治疗途径．方法：实验选用恒河猴１２只，于腰４５行异体椎间盘移植手术．对术后不同时间组移植椎间盘进行３ＨＰｒｏｌｉｎｅ同位素标记衰变计数组织学活性及生物化学组成成分变化的观察．结果：移植椎间盘髓核组织术后６ｍｏ及纤维环组织术后３ｍｏ始３ＨＰｒｏｌｉｎｅ掺入量增加．髓核水含量改变不明显，糖醛酸１２ｍｏ组较对照组降低（Ｐ＜０．０５），３，６，９ｍｏ组的羟脯氨酸含量均较对照组明显增高（Ｐ＜０．０５）．纤维环的水份含量在９ｍｏ组较对照组显著降低；糖醛酸含量９ｍｏ组降低，羟脯氨酸含量术后６月增加，９ｍｏ～１２ｍｏ又逐渐降低．结论：①移植间盘术后其组织内存有活性细胞成分，活性细胞在移植间盘营养通道恢复后一定时间期限内数量增多并代谢活性升高．②移植间盘术后出现一定程度的退变，但同时移植间盘显示出一定的增生修复能力．Aim: To explore the survival of fresh disc allograft and the change of disc viability and biochemistry in a fresh allograft. Methods: Under general anaesthesia, osteotomies were made at the endplates about 2.0 mm away from the L45 intervertibral disc,then the fresh discs were transposed in two matched monkeys. The four groups of animals were sacrificed after 3,6,9 and 12 months respectively. Cell viability was assessed by measuring 3HProline incorporation and biochemical changes were measured by water,proteoglycan and collagen. Results: Disc viability study demonstrated that the 3HProline DPM(disintegration per minute) increased in the allografted discs. In the nucleus pulposus of implanted disc biochemical study showed that proteoglycan contents were not changed obviously after 9 months, but proteoglycan content decreased at the 12th month. Collagen content increased at 3,6,9 months. There was not change of water among twelve months. In the annulus fibrous of implanted disc, proteoglycan and water contents decreased at the 9th month,but they were increased at the 12th month. Collagen content increased at the 6th month,but it decreased at 9th month again. Conclusion: The present results showed the intervertibral disc grafted survived allologous transplantation. The allografted intervertibral disc appeared some ability of regeneration.

关 键 词：恒河猴 异体 椎间盘移植 蛋白多糖 生物学活性 

分 类 号：R681.530.5[医药卫生—骨科学]