低氧对大鼠骨髓间质干细胞的影响  

Effects of Low Oxygen Tension on the Proliferation and Adhesion of Mouse MSCs

作　　者：朱斌[1] 顾春虎[2] 宿学家[1] 张志勇[1] 易定华[2] 

机构地区：[1]解放军第八十八医院心脏外科 [2]第四军医大学西京医院心脏外科,西安710032

出　　处：《中国现代医生》2009年第2期34-36,F0003,共4页China Modern Doctor

基　　金：国家自然科学基金资助项目(30600137);陕西省攻关课题[2005k11-G1(2)];国家"863"计划重点资助项目(2006AA02A138)

摘　　要：目的探讨低氧培养对大鼠骨髓间充质干细胞(MSCs)增殖、粘附及分泌的影响。方法通过细胞增殖实验、细胞粘附实验及Western-blotting,分别观察大鼠MSCs在低氧(6%O2)和常氧(21%O2)的增殖、粘附,及纤连蛋白(FN)和玻璃粘连蛋白(VN)的表达。结果低氧组10d内培养即可扩增到(1.3±0.12)×105细胞/孔,明显高于常氧组((0.68±0.06)×105)细胞/孔(P<0.05);低氧组24h细胞粘附率((89.14±2.4)%)高于常氧组((62.13±2.6)%)(P<0.05),同时FN和VN的蛋白表达显著增加(P<0.05)。结论低氧作为体外的一种可控制因素,调节MSCs的增殖及粘附功能,对于MSCs的组织工程的应用具有一定的意义。Objective To investigate the effects of low oxygen tension on the proliferation,adhesion and secretion of mouse mesenchymal stem cells （MSCs）. Methods Proliferation test, adhesion assay and western-blotting were performed to observe proliferation, adhesion, and the protein expression of fibronectin and vitroneetin of mouse MSCs ,which cultured in 6%O2 and 21%O2. Results Compared to 21%O2, （1.3 ± 0.12） × 10^5 cells/well could be obtained after 10 days culture in 6%O2 （P〈 0.05）. Ration of 24h adhesion in 6%02 group （ （89.14 ± 2.4）% ） were signieantly higher than that in 21%O2 group（ （62.13 ± 2.6）% ）（ P 〈 0.05 ）. The protein expression of FN and VN were also higher in 6%O2 group（P 〈 0.05 ）. Conclusion Low oxygen tension as a vitro factor which can be controlled in vitro can regulate MSCs in proliferation, adhesion and extracellular matrix secretion, and have certain significance in the applying of MSCs in tissue engineering.

关 键 词：低氧 增殖 粘附 骨髓 间充质干细胞 大鼠 纤连蛋白 

分 类 号：R331.2[医药卫生—人体生理学]