决明子和山楂组分配伍对兔肝细胞膜高密度脂蛋白受体活性的影响  

Effect of Combination of Anthraquinone Glucosides from Cassia Seed and Shanzha Total Triterpenoid Acid on Activity of High Density Lipoprotein Receptor in Hyperlipidemic Rabbits

作　　者：马路[1] 江梦溪[2] 刘剑刚[3] 史大卓[3] 陈可冀[3] 苗明三[4] 

机构地区：[1]中国人民解放军海军总医院,北京100037 [2]郑州大学药学院,河南郑州450052 [3]中国中医科学院西苑医院,北京100091 [4]河南中医学院药学院,河南郑州450008

出　　处：《中国实验方剂学杂志》2009年第1期24-28,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中国博士后基金资助项目(2004035408)

摘　　要：目的:观察决明子蒽醌苷与山楂总三萜酸配伍对实验性高血脂症兔血清低密度脂蛋白胆固醇(low densit ylipoprotein-cholesterol LDL-C)及肝细胞膜高密度脂蛋白受体(high density lipoprotein receptor HDLR)活性的影响。方法:雄性新西兰兔高脂饲养2周形成实验性高血脂症,继续给予高脂饮食,然后采用均匀设计法,分别给予决明子和山楂有效部位不同的比例配伍灌服,4周后高脂给量减半,继续服药至6周,免疫比浊法测定血清LDL-C变化,以125I标记的兔HDL3作为配体,用放射受体分析法对肝细胞膜HDLR活性进行测定。结果:决明子蒽醌苷与山楂总三萜酸按1∶2.63配伍,可使血清LDL-C含量明显降低(P<0.01),肝细胞膜HDLR活性增加(P<0.05)。结论:决明子蒽醌苷与山楂总三萜酸最佳配比降低高血脂兔血清LDL-C的作用明显强于单一组分及原药对配伍,对肝细胞膜HDLR的影响呈现以受体数目显著增加为特征的受体结合活性升高。Objective： To observe the effect of combination of Anthraquinone Glucosides from Cassia Seed （Agc） and Shanzha total triterpenoid acids （Sta） on low density lipoprotein-cholesterol （LDL-C）of serum and activity of high density lipoprotein receptor （HDLR） in hyperlipidemic rabbits. Methords： The best proportion of Agc and Sta was determined by uniform design as the strongest effect on decreasing LDL-C of serum. The best proportional combination of Agc and Sta was given to hyperlipidemic rabbits. Activity of HDLR on the hepatic plasma membranes was measured with ^125I.-labeled HDL3 as ligand. Results： The results showed that the combination could markedly decrease the level of LDL-C of serum and increase the activity of HDLR at the proportion： Agc ： Sta = 1 ： 2.63. Conclusion： For the best proportion, the combined effect of decreasing in LDL-C was stronger than Agc or Sta or the original prescription. The combination could enhance the activity of ,HDLR by increasing the number of HDLR.

关 键 词：决明子蒽醌苷 山楂总三萜酸 高密度脂蛋白受体 均匀设计 

分 类 号：R285.5[医药卫生—中药学]