昆虫病原线虫(Steinernema longicaudum BPS品系)生物学测定  

Bio-Analysis on EPNs of Steinernema longicaudum BPS Strain

作　　者：刘奇志[1] 张丽娟[1] 王玉柱[2] 杜小康[1] 孙浩元[2] Zdeneck Mráek 

机构地区：[1]中国农业大学农学与生物技术学院昆虫系昆虫与线虫学实验室,北京100193 [2]北京市农林科学院林业果树研究所,北京100093 [3]捷克科学院昆虫研究所生物学中心

出　　处：《中国农学通报》2009年第2期236-239,共4页Chinese Agricultural Science Bulletin

基　　金：国家科技部863课题"昆虫线虫修复生物污染土壤技术探索"(2006AA06Z354);国家科技支撑计划项目"主要果树高效标准生产技术研究"(2008BAD92B08);北京市政府购买科技服务项目(2007DA903B03-07)

摘　　要：研究旨在明确采自北京平谷圣泉庵橡树根际土壤中昆虫病原线虫(Steinernema longicaudum BPS)的生物学特性,进而明确开发应用的可能性。结果显示:在剂量10和20IJs/幼虫下,BPS对寄主幼虫的侵染效果相近,大蜡螟的校正死亡率分别为40.0%和36.7%,黄粉甲的分别为50.0%、53.3%;在剂量40、80、160IJs/幼虫下,BPS的侵染力随剂量的增高而增大,大蜡螟和黄粉甲的校正死亡率分别为73.3%、86.7%、90.0%和70.0%、80.0%、90.0%;在剂量320和640IJs/幼虫下的侵染效果(校正死亡率100%)相近。BPS经40℃水浴处理1h校正死亡率达98%,处理3h,线虫100%死亡;经37℃、36℃和34℃水浴处理10h,校正死亡率分别为92.4%、55.4%和45.7%,处理20h,线虫100%死亡。该线虫在30℃条件下的水平运动能力比在18℃和25℃的强,有34.6%的线虫用24h的时间运动到3.6cm处。研究结果表明BPS具有较强的侵染力、耐热性和水平扩散能力,据此初步分析BPS具有被开发应用潜能。The biology analysis on EPNs of Steinernema longicaudum BPS strain, isolated from the oak tree soil near Sheng Quan nunnery in Pinggu district of Beijing, China, was studied to understand the potential of being bio-control agent. The results shown that after 72 h infection at 25 ℃, BPS had similar effectivity to infect hosts at the dosages of 10 and 20 IJs/Iarva. The proofreaded mortality （PM） of Galleria mellonella and Tenebrio molitor was 40.0%, 36.7% and 50.0%, 53.3%, respectively. The ability of BPS infection at the dosages of 40, 80 and 160 IJs/larva was increased while the dosages were raised up. The PM of G. mellonella and T. molitor was 73.3%, 86.7%, 90.0% and 70.0%, 80.0%, 90.0%, respectively. BPS infection at the dosages of 320 and 640 IJs/larva had same effect （100% PM） to both hosts. The heat tolerance of BPS at 40℃ for lh and 3 h, the PM was 98% and 100%, respectively. It was 92.4%, 55.4% and 45.7%, respectively at 37, 36 and 34 ~C for 10 h, and 100% for 20h. BPS ability of transverse diffusion at 30℃ was better than that at 18℃ and 25℃. There was 34.6% individuals could move 3.6 cm in 24 h at 30℃. The results indicated that BPS had good capacity of host infection, heat tolerance and transverse diffusion. Hereby, it was concluded that BPS has the potential to development and application.

关 键 词：BPS线虫 生物学 侵染力 耐热性 水平扩散 

分 类 号：S476.15[农业科学—农业昆虫与害虫防治]