日本血吸虫组织蛋白酶的研究  

STUDIES ON CATHEPSINS IN SCHISTOSOMA JAPONICUM *

作　　者：李雍龙[1,2] 冯友仁[1,2] 

机构地区：[1]同济医科大学寄生虫学教研室 [2]德国海德堡大学热带医学研究所

出　　处：《中国寄生虫学与寄生虫病杂志》1998年第2期101-104,共4页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：卫生部资助

摘　　要：目的：鉴别日本血吸虫组织蛋白酶类型及其水解蛋白活性。方法：收集日本血吸虫成虫肠道排出物，用含精氨酰键的特异性人工合成底物识别排出物中组织蛋白酶的类型及活性。结果：成虫肠道排出物能降解组织蛋白酶Ｂ的特异性底物７－氨基－４－甲基香豆素（Ｚ－Ａｒｇ－Ａｒｇ－ＡＭＣ）和组织蛋白酶Ｂ及Ｌ的共同底物Ｚ－Ｐｈｅ－Ａｒｇ－ＡＭＣ。组织蛋白酶Ｌ的抑制剂Ｚ－Ｐｈｅ－Ｐｈｅ－ＣＨＮ２能部分抑制排出物对Ｚ－ｐｈｅ－Ａｒｇ－ＡＭＣ的降解作用。日本血吸虫组织蛋白酶Ｂ／Ｌ的最适ｐＨ为５．０－５．５。结论：日本血吸虫成虫排出物具有组织蛋白酶Ｂ和Ｌ样活性。AIM:To identify the cathepsins in adult worms of Schistosoma japonicum and their proteolytic activities. METHODS: The vomitus from adult worms of S. japonicum was collected. The proteolytic activity of cathepsins in the vomitus was identified and measured using synthetic substrates with arginyl bonds. RESULTS: Specific synthetic substracts Z Arg Arg AMC (for cathepsin B) and Z Phe Arg AMC (for cathepsin B and L) were degraded by the vomitus. An inhibitor, Z Phe PheCHN 2 for cathepsin L, inhibited partialy the activity of the vomitus to degrade Z Phe Arg AMC. The optimal pH for cathepsin B/L is 5 0-5 5.CONCLUSION: The vomitus of S.japonicum has cathepsin B and L like activity.

关 键 词：日本血吸虫 组织蛋白酶 蛋白水解 

分 类 号：R383.24[医药卫生—医学寄生虫学]