雷丸蛋白酶体外抗猪囊尾蚴组织学变化的观察  

HISTOLOGICAL CHANGES OF CYSTICERCUS CELLULOSAE UNDER THE ACTION OF PROTEINASE OF OMPHALIA LAPIDESCENS IN VITRO

作　　者：赵冠宏[1] 许炽标[1] 冯曼玲[1] 尤金英[1] 郭毛娣[2] 李焕娄[2] 

机构地区：[1]北京热带医学研究所 [2]中国医学科学院医药生物技术研究所

出　　处：《中国寄生虫学与寄生虫病杂志》1998年第2期113-116,共4页Chinese Journal of Parasitology and Parasitic Diseases

摘　　要：目的：寻找一种高效、低毒和价廉的杀虫药物，特别是在中草药中发掘有效杀囊虫药。方法：采用人工发酵雷丸菌（ＯｍｐｈａｌｉａｌａｐｉｄｅｓｃｅｎｓＳｃｈｒｏｅｔｅｒ）蛋白酶进行体外杀囊虫试验，并制成石蜡切片，Ｈ．Ｅ．染色后，作镜检，观察其组织结构的变化情况。结果：雷丸蛋白酶对猪囊尾蚴大体形态、组织结构均有明显的破坏作用，可侵入实质细胞层。结论：证实了雷丸蛋白酶是杀囊虫的有效成分。AIM: For the purpose of finding an anthelmintic with high efficacy,low toxicity and low cost, especially from the Chinese tranditional medicines. METHODS:Forty Cysticercus cellulosae were separately incubated with proteinase from artificially fermented Omphalia lapidescens for 2 h, 4 h and 8 h, and then examined for histological changes by light microscopy and compared with those of proteinase from natural dry Omphalia lapidescens after similar treatment.RESULTS: Cysticercus cellulosae were morphologically and structurally impaired after exposure to the action of proteinase of Omphalia lapidescens ,and the degree of impairment was proportionally paralleled to the duration of treatment. CONCLUSION:The anti cysticercus effect of proteinase of Omphalia lapidescens and the homogeneity of proteinases from artificially fermented and natural Omphalia lapidescens were confirmed.

关 键 词：发酵雷丸蛋白酶 猪囊尾蚴 中药药理学 

分 类 号：R285.5[医药卫生—中药学] R259.323.3[医药卫生—中医学]